NDUFA5 Antibody (4A2) - Azide and BSA Free

Western Blot: NDUFA5 Antibody (4A2) [H00004698-M02] - Analysis of NDUFA5 expression in transfected 293T cell line by NDUFA5 monoclonal antibody (M02), clone 4A2.Lane 1: NDUFA5 transfected lysate(13.5 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: NDUFA5 Antibody (4A2) [H00004698-M02] - Analysis of monoclonal antibody to NDUFA5 on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.

Western Blot: NDUFA5 Antibody (4A2) [H00004698-M02] - NDUFA5 monoclonal antibody (M02), clone 4A2. Analysis of NDUFA5 expression in human kidney.

Sandwich ELISA: NDUFA5 Antibody (4A2) [H00004698-M02] - Detection limit for recombinant GST tagged NDUFA5 is 10 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 4A2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NDUFA5 Antibody (4A2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: NDUFA5 (NP_004991.1, 1 a.a. ~ 116 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAGVLKKTTGLVGLAVCNTPHERLRILYTKILDVLEEIPKNAAYRKYTEQITNEKLAMVKAEPDVKKLEDQLQGGQLEEVILQAEHELNLARKMREWKLWEPLVEEPPADQWKWPI
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NDUFA5
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
• Application Notes: It has been used for ELISA and WB.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NDUFA5 Antibody (4A2) - Azide and BSA Free

• B13,5 (13kD, B13)
• DKFZp781K1356
• NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa
• UQOR13FLJ12147

Background

The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble "iron-sulfur protein" (IP) fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDUFA5
• Uniprot:
  • NP_004991(Human)