NCK1 Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NCK1 Antibody Summary

Immunogen
This antibody was made against a protein fragment from the Middle Region
Specificity
This product is specific for Human NCK1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCK1
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250-1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
This antibody is useful in ELISA, Western Blot, Immunohistochemistry-Paraffin and Immunoprecipitation.
Control
NCK1 Lysate (NBP2-66183)
Publications
Read Publications using 24800002.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

Manufactured by SDIX's proprietary Genomic Antibody Technology™. GAT FAQs. These products are available in limited quantities. Please enquire about the availability of this product before purchasing.

Alternate Names for NCK1 Antibody

  • cytoplasmic protein NCK1
  • MGC12668
  • NCK adaptor protein 1melanoma NCK protein
  • NCK1
  • nck-1
  • NCKalpha
  • NCKNCK tyrosine kinase
  • non-catalytic region of tyrosine kinase
  • SH2/SH3 adaptor protein NCK-alpha

Background

Nck is a ubiquitously expressed adaptor protein consisting of one SH2 and three SH3 Src homology domains. Stimulation by epidermal growth factor (EGF) or platelet-derived growth factor (PDGF) leads to phosphorylation of Nck on tyrosine, serine and threonine residues. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast (1-2). Additionally, Nck has been shown to associate with BLNK, PLCG1 and VAV1 in a B-cell antigen receptor-dependent fashion and may play a role in linking tyrosine and serine/threonine kinase pathways within the cell (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NCK1 Antibody (24800002)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for NCK1 Antibody (24800002) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Diseases for NCK1 Antibody (24800002)

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Bioinformatics

Gene Symbol NCK1
Entrez
Uniprot