NCK1 Antibody (JU41-80)

Images

 
Western Blot: NCK1 Antibody (JU41-80) [NBP2-75585] - Analysis of NCK1 on different lysates using anti-NCK1 antibody at 1/500 dilution.Positive control:Lane 1: Hela Lane 1: NIH-3T3Lane 2: Rat kidney ...read more
Immunocytochemistry/ Immunofluorescence: NCK1 Antibody (JU41-80) [NBP2-75585] - Staining NCK1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: NCK1 Antibody (JU41-80) [NBP2-75585] - Analysis of paraffin-embedded rat testis tissue using anti-NCK1 antibody. Counter stained with hematoxylin.
Flow Cytometry: NCK1 Antibody (JU41-80) [NBP2-75585] - Analysis of Jurkat cells with NCK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). ...read more
Immunocytochemistry/ Immunofluorescence: NCK1 Antibody (JU41-80) [NBP2-75585] - Staining NCK1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: NCK1 Antibody (JU41-80) [NBP2-75585] - Staining NCK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: NCK1 Antibody (JU41-80) [NBP2-75585] - Analysis of paraffin-embedded human tonsil tissue using anti-NCK1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: NCK1 Antibody (JU41-80) [NBP2-75585] - Analysis of paraffin-embedded human kidney tissue using anti-NCK1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: NCK1 Antibody (JU41-80) [NBP2-75585] - Analysis of paraffin-embedded mouse testis tissue using anti-NCK1 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
JU41-80
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

NCK1 Antibody (JU41-80) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant protein
Localization
Cytoplasm. Endoplasmic reticulum. Nucleus.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NCK1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10 - 1:500
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for NCK1 Antibody (JU41-80)

  • cytoplasmic protein NCK1
  • MGC12668
  • NCK adaptor protein 1melanoma NCK protein
  • NCK1
  • nck-1
  • NCKalpha
  • NCKNCK tyrosine kinase
  • non-catalytic region of tyrosine kinase
  • SH2/SH3 adaptor protein NCK-alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NCK1