NCK1 Antibody (JU41-80)

Western Blot: NCK1 Antibody (JU41-80) [NBP2-75585] - Analysis of NCK1 on different lysates using anti-NCK1 antibody at 1/500 dilution.Positive control: Lane 1: Hela Lane 2: NIH-3T3 Lane 2: Rat kidney tissueLane 3: Mouse testis tissue

Immunocytochemistry/Immunofluorescence: NCK1 Antibody (JU41-80) [NBP2-75585] - Staining NCK1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: NCK1 Antibody (JU41-80) [NBP2-75585] - Analysis of paraffin-embedded rat testis tissue using anti-NCK1 antibody. Counter stained with hematoxylin.

Flow Cytometry: NCK1 Antibody (JU41-80) [NBP2-75585] - Analysis of Jurkat cells with NCK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: NCK1 Antibody (JU41-80) [NBP2-75585] - Staining NCK1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: NCK1 Antibody (JU41-80) [NBP2-75585] - Staining NCK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: NCK1 Antibody (JU41-80) [NBP2-75585] - Analysis of paraffin-embedded human tonsil tissue using anti-NCK1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: NCK1 Antibody (JU41-80) [NBP2-75585] - Analysis of paraffin-embedded human kidney tissue using anti-NCK1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: NCK1 Antibody (JU41-80) [NBP2-75585] - Analysis of paraffin-embedded mouse testis tissue using anti-NCK1 antibody. Counter stained with hematoxylin.

Product Discontinued

NCK1 Antibody (JU41-80) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human NCK1 aa 204-296 / 377. (SwissProt: P16333 Human; SwissProt: Q99M51 Mouse; SwissProt: B2RZ33 Rat)
• Localization: Cytoplasm. Endoplasmic reticulum. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NCK1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for NCK1 Antibody (JU41-80)

• cytoplasmic protein NCK1
• MGC12668
• NCK adaptor protein 1melanoma NCK protein
• NCK1
• nck-1
• NCKalpha
• NCKNCK tyrosine kinase
• non-catalytic region of tyrosine kinase
• SH2/SH3 adaptor protein NCK-alpha

Background

Nck is a ubiquitously expressed adaptor protein consisting of one SH2 and three SH3 Src homology domains. Stimulation by epidermal growth factor (EGF) or platelet-derived growth factor (PDGF) leads to phosphorylation of Nck on tyrosine, serine and threonine residues. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast (1-2). Additionally, Nck has been shown to associate with BLNK, PLCG1 and VAV1 in a B-cell antigen receptor-dependent fashion and may play a role in linking tyrosine and serine/threonine kinase pathways within the cell (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCK1