Human NANS ELISA Kit (Colorimetric)

ELISA: Human NANS ELISA Kit (Colorimetric) [NBP3-18741] - Standard Reference Curve

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Tissue Extract
• Standard Curve Range: 1 - 64 ng/mL (example only; lot dependent)
• Sensitivity: 0.49 ng/mL (example only; lot dependent)

Human NANS ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 1 - 64 ng/mL (example only; lot dependent)
• Sensitivity: 0.49 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: CV%< 10.1 (example only; lot dependent)
• Intra-Assay: CV%< 5.2 (example only; lot dependent)
• Spike Recovery: 105% (example only; lot dependent)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NANS

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human NANS ELISA Kit (Colorimetric)

• EC 2.5.1.56
• EC 2.5.1.57
• N-acetylneuraminate synthase
• N-acetylneuraminate-9-phosphate synthase
• N-acetylneuraminic acid phosphate synthase
• N-acetylneuraminic acid synthasesialic acid phosphate synthase
• SASsialic acid synthase

Background

NANS encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for NANS ELISA Kit (NBP3-18741). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NANS