NAALADase-2/NAALAD2 Antibody (756030) [Unconjugated]

Western blot shows lysates of human testis tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human NAALADase-2/NAALAD2 Monoclonal Antibody (Catalog # MAB7658) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NAALADase-2/NAALAD2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

NAALADase-2/NAALAD2 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human NAALADase-2/ NAALAD2 Monoclonal Antibody (Catalog # MAB7658) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 756030
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NAALADase-2/NAALAD2 Antibody (756030) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human NAALADase-2/NAALAD2
  Lys32-Leu740
  Accession # Q9Y3Q0
• Specificity: Detects human NAALADase-2/NAALAD2 in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse NAALADase-2 is observed and no cross-reactivity with recombinant human (rh) PSMA/FOLH1/NAALADase-1, rhNAALADase-like 2, or rhNAALADase-like 1 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NAALAD2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NAALADase-2/NAALAD2 Antibody (756030) [Unconjugated]

• GCPIII
• Glutamate Carboxypeptidase III
• GPCIII
• MGC116996
• MGC26353
• NAADALASE2
• NAALAD2
• NAALADase II
• NAALADase2
• NAALADase-2
• N-acetylated alpha-linked acidic dipeptidase 2
• N-acetylated alpha-linked acidic dipeptidase II
• N-acetylated-alpha-linked acidic dipeptidase 2
• N-acetylated-alpha-linked acidic dipeptidase II

Background

N-acetylated-alpha-linked acidic dipeptidase 2 (NAALADase-2), also known as glutamate carboxypeptidase 3 (GCP3), is a 90-100 kDa type 2 transmembrane protein in the M28 peptidase family. It hydrolyzes beta-citrylglutamate (BCG) and N-acetyl-aspartylglutamate (NAAG) which are abundant dipeptides found in brain and testis. Mature human NAALADase-2 consists of a 7 amino acid (aa) cytoplasmic domain, a 24 aa transmembrane segment, and a 709 aa extracellular region (aa 32-740) that contains the peptidase domain. Over aa 32-740, human NAALADase-2 shares 89% aa sequence identity with mouse NAALADase-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAALAD2
• Uniprot:
  • Q9Y3Q0(Human)