Amisyn Antibody

Immunohistochemistry: Amisyn Antibody [NB100-1227] - (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Amisyn Antibody Summary

• Immunogen: Peptide with sequence SAKSAISKEIFAP-C corresponding to N-Terminus according to NP_054897.4.
• Localization: Cytoplasmic
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: STXBP6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot
• Application Notes: WB: Preliminary experiments gave bands at both approx. 25 kDa band and approx. 45 kDa band in human brain extracts at 0.1 ug/ml. Please note that currently we cannot find an explanation in scientific literature for the 45 kDa band we observe given the predicted MW of approx. 24 kDa band according to NP_054897. IHC-P: Human kidney shows cytoplasm staining of PCT and DCT cells.Use in Western blot reported in scientific literature (PMID: 24441682).
• Control:

  Amisyn Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24441682).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Amisyn Antibody

• amisyn
• FLJ39638
• HSPC156
• syntaxin binding protein 6 (amisyn)
• syntaxin-binding protein 6

Background

STXBP6 binds components of the SNARE complex (see MIM 603215) and may be involved in regulating SNARE complex formation (Scales et al., 2002).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Amisyn Antibody (NB100-1227)(2)

Publications using NB100-1227

• Huang TN, Chuang HC, Chou WH et al. Tbr1 haploinsufficiency impairs amygdalar axonal projections and results in cognitive abnormality. Nat. Neurosci. 2014-02-01 [PMID: 24441682] (WB, Mouse)
• Scales SJ, Hesser BA, Masuda ES, Scheller RH. Amisyn, a novel syntaxin-binding protein that may regulate SNARE complex assembly. J Biol Chem 2002-08-02 [PMID: 12145319]

Bioinformatics

• Gene Symbol: STXBP6
• Entrez:
  • 29091(Human)
  • 217517(Mouse)
• Uniprot:
  • Q8NFX7(Human)