![Western Blot: N4BP1 Antibody [NBP2-37688] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-N4BP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/N4BP1-Antibody-Western-Blot-NBP2-37688-img0001.jpg "Western Blot: N4BP1 Antibody [NBP2-37688] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-N4BP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | This antibody maps to a region between residue 250 to 300 of human NEDD4 Binding Protein 1 using the numbering given in entry NP_694574.3 (GeneID 9683). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | N4BP1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP2-37688 | Applications | Species |
|---|---|---|
| Saha B, Salemi M, Williams GL et al. Interactomic analysis reveals a homeostatic role for the HIV restriction factor TRIM5 alpha in mitophagy Cell reports 2022-05-10 [PMID: 35545034] |
Secondary Antibodies |
Isotype Controls |
Research Areas for N4BP1 Antibody (NBP2-37688)Find related products by research area.
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| Gene Symbol | N4BP1 |
| Uniprot |
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![Immunoprecipitation: N4BP1 Antibody [NBP2-37688] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-N4BP1 antibody used for IP at 6 ug per reaction. N4BP1 was also immunoprecipitated by rabbit anti-N4BP1 antibody BL17672. For blotting immunoprecipitated N4BP1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/N4BP1-Antibody-Immunoprecipitation-NBP2-37688-img0002.jpg "Immunoprecipitation: N4BP1 Antibody [NBP2-37688] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Affinity purified rabbit anti-N4BP1 antibody used for IP at 6 ug per reaction. N4BP1 was also immunoprecipitated by rabbit anti-N4BP1 antibody BL17672. For blotting immunoprecipitated N4BP1, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
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![Chromatin Immunoprecipitation (ChIP) SOX17 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX17_AF1924_Chromatin_Immunoprecipitation_11220.jpg)
![Simple Western SOX17 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX17_AF1924_Simple_Western_16675.jpg)
![Immunocytochemistry SOX17 Antibody [Unconjugated]](https://images.novusbio.com/images2/SOX17_AF1924_Immunocytochemistry_6477.jpg)
![Immunocytochemistry CKAP4/p63 Antibody [Unconjugated]](https://images.novusbio.com/images2/CKAP4_AF7355_Immunocytochemistry__Immunofluorescence_17000.jpg)
![Western Blot CKAP4/p63 Antibody [Unconjugated]](https://images.novusbio.com/images2/CKAP4_AF7355_Western_Blot_11997.jpg)
![Immunocytochemistry CKAP4/p63 Antibody [Unconjugated]](https://images.novusbio.com/images2/CKAP4_AF7355_Immunocytochemistry_12002.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")