N-WASP Antibody (JU07-31)

Western Blot: N-WASP Antibody (JU07-31) [NBP2-75599] - Analysis of WASL on mouse Human serum lysates using anti-Ubiquitin antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: N-WASP Antibody (JU07-31) [NBP2-75599] - Staining WASL in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: N-WASP Antibody (JU07-31) [NBP2-75599] - Analysis of paraffin-embedded mouse brain tissue using anti-WASL antibody. Counter stained with hematoxylin.

Flow Cytometry: N-WASP Antibody (JU07-31) [NBP2-75599] - Analysis of K562 cells with WASL antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Immunocytochemistry/Immunofluorescence: N-WASP Antibody (JU07-31) [NBP2-75599] - Staining WASL in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: N-WASP Antibody (JU07-31) [NBP2-75599] - Analysis of paraffin-embedded human spleen tissue using anti-WASL antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: N-WASP Antibody (JU07-31) [NBP2-75599] - Analysis of paraffin-embedded human kidney tissue using anti-WASL antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: N-WASP Antibody (JU07-31) [NBP2-75599] - Analysis of paraffin-embedded human colon cancer tissue using anti-WASL antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: N-WASP Antibody (JU07-31) [NBP2-75599] - Analysis of paraffin-embedded mouse colon tissue using anti-WASL antibody. Counter stained with hematoxylin.

Product Discontinued

N-WASP Antibody (JU07-31) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human N-WASP aa 401-450 / 505. (SwissProt: O00401 Human; SwissProt: Q91YD9 Mouse)
• Localization: Cytoplasm, cytoskeleton. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: WASL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for N-WASP Antibody (JU07-31)

• NWASP
• N-WASP
• N-WASPneural WiskNWASP
• TRS4
• WASL
• WiskDKFZp779G0847
• WiskMGC48327

Background

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: WASL