N-Acetylglucosaminyltransferase V/MGAT5 Antibody (3E9) - Azide and BSA Free

Western Blot: N-Acetylglucosaminyltransferase V/MGAT5 Antibody (3E9) [H00004249-M09] - Analysis of MGAT5 expression in Jurkat (Cat # L017V1).

Immunohistochemistry-Paraffin: N-Acetylglucosaminyltransferase V/MGAT5 Antibody (3E9) [H00004249-M09] - Analysis of monoclonal antibody to MGAT5 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml

ELISA: N-Acetylglucosaminyltransferase V/MGAT5 Antibody (3E9) [H00004249-M09] - N-Acetylglucosaminyltransferase V/MGAT5 Antibody (3E9) [H00004249-M09] - Detection limit for recombinant GST tagged MGAT5 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 3E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

N-Acetylglucosaminyltransferase V/MGAT5 Antibody (3E9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MGAT5 (NP_002401, 642 a.a. ~ 739 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LAEPGQSCKQVCQESQLICEPSFFQHLNKDKDMLKYKVTCQSSELAKDILVPSFDPKNKHCVFQGDLLLFSCAGAHPRHQRVCPCRDFIKGQVALCKD
• Specificity: Reacts with mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MGAT5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Antibody reactivity against cell lysate in WB and recombinant protein in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for N-Acetylglucosaminyltransferase V/MGAT5 Antibody (3E9) - Azide and BSA Free

• alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
• Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase
• EC 2.4.1.155
• GGNT5
• GlcNAc-T V
• GNT-VGNT-VA
• Mannoside acetylglucosaminyltransferase 5
• mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase
• MGAT5
• N-Acetylglucosaminyltransferase V
• N-acetylglucosaminyl-transferase V

Background

This gene encodes mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, a glycosyltransferase involved in the synthesis of protein-bound and lipid-bound oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the encoded protein's activity may correlate with the progression of invasive malignancies. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for N-Acetylglucosaminyltransferase V/MGAT5 Antibody (H00004249-M09)(1)

Publication using H00004249-M09

• Kippe JM, Mueller TM, Haroutunian V, Meador-Woodruff JH. Abnormal N-acetylglucosaminyltransferase expression in prefrontal cortex in schizophrenia Schizophr. Res. 2015-06-20 [PMID: 26104473] (WB, Human)

Bioinformatics

• Gene Symbol: MGAT5
• Entrez:
  • 4249(Human)
• Uniprot:
  • NP_002401(Human)