N acetyl transferase 5 Antibody (2C6)

Western Blot: N acetyl transferase 5 Antibody (2C6) [H00051126-M01] - NAT5 monoclonal antibody (M01), clone 2C6 Analysis of NAT5 expression in HL-60.

Sandwich ELISA: N acetyl transferase 5 Antibody (2C6) [H00051126-M01] - Detection limit for recombinant GST tagged NAT5 is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IP, S-ELISA
• Clone: 2C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

N acetyl transferase 5 Antibody (2C6) Summary

• Immunogen: NAT5 (AAH05181, 1 a.a. ~ 178 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTTLRAFTCDDLFRFNNINLDPLTETYGIPFYLQYLAHWPEYFIVAEAPGGELMGYIMGKAEGSVAREEWHGHVTALSVAPEFRRLGLAAKLMELLEEISERKGGFFVDLFVRVSNQVAVNMYKQLGYSVYRTVIEYYSASNGEPDEDAYDMRKALSRDTEKKSIIPLPHPVRPEDIE
• Specificity: NAT5 - N-acetyltransferase 5 (ARD1 homolog, S. cerevisiae)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NAA20
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for N acetyl transferase 5 Antibody (2C6)

• dJ1002M8.1
• dJ1175I6.2 (N-terminal acetyltransferase complex ard1 subunit)
• EC 2.3.1.88
• homolog)
• N(alpha)-acetyltransferase 20, NatB catalytic subunit
• N-acetyltransferase 3 homolog
• N-acetyltransferase 5 (ARD1 homolog, S. cerevisiae)
• N-acetyltransferase 5 (GCN5-related, putative)
• N-acetyltransferase 5
• N-acetyltransferase 5, ARD1 subunit (arrest-defective 1, S. cerevisiae
• N-alpha-acetyltransferase 20, NatB catalytic subunit
• NAT3N-terminal acetyltransferase B complex catalytic subunit NAT5
• NAT5dJ1002M8.1 (N-terminal acetyltransferase complex ard1 subunit)
• NatB complex subunit NAT5
• N-terminal acetyltransferase B complex catalytic subunit NAA20
• N-terminal acetyltransferase complex ARD1 subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for N acetyl transferase 5 Antibody (H00051126-M01)(2)

Publications using H00051126-M01

• Van Damme P, Lasa M, Polevoda B et al. N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB. Proc Natl Acad Sci U S A. 2012-07-18 [PMID: 22814378]
• Starheim KK, Arnesen T, Gromyko D et al. Identification of the human Nalpha-acetyltransferase complex B (hNatB) - a complex important for cell cycle progression. Biochem J;415(2):325-31. 2008-10-15 [PMID: 18570629]

Bioinformatics

• Gene Symbol: NAA20
• Entrez:
  • 51126(Human)
• Uniprot:
  • AAH05181(Human)