N-Acetyl-D-Glucosamine Kinase/NAGK Recombinant Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

N-Acetyl-D-Glucosamine Kinase/NAGK Recombinant Protein Summary

Description
A recombinant protein with a His-tag corresponding to amino acids 1 to 344 of Human N-Acetyl-D-Glucosamine Kinase/NAGK

Source: E. coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAIYGG VEGGGTRSEV LLVSEDGKIL AEADGLSTNH WLIGTDKCVE RINEMVNRAK RKAGVDPLVP LRSLGLSLSG GDQEDAGRIL IEELRDRFPY LSESYLITTD AAGSIATATP DGGVVLISGT GSNCRLINPD GSESGCGGWG HMMGDEGSAY WIAHQAVKIV FDSIDNLEAA PHDIGYVKQA MFHYFQVPDR LGILTHLYRD FDKCRFAGFC RKIAEGAQQG DPLSRYIFRK AGEMLGRHIV AVLPEIDPVL FQGKIGLPIL CVGSVWKSWE LLKEGFLLAL TQGREIQAQN FFSSFTLMKL RHSSALGGAS LGARHIGHLL PMDYSANAIA FYSYTFS
Preparation
Method
E.coli
Gene
NAGK
Purity
>95% pure by SDS-PAGE

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200mM NaCl
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Application Notes
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 39.8kDa (367aa), confirmed by MALDI-TOF

Reactivity Notes

Human

Alternate Names for N-Acetyl-D-Glucosamine Kinase/NAGK Recombinant Protein

  • EC 2.7.1.59
  • GlcNAc Kinase
  • GNK
  • GNKGlcNAc kinase
  • N-acetyl-D-glucosamine kinase
  • N-Acetylglucosamine Kinase
  • N-acetylglucosamine kinaseHSA242910
  • NAGK

Background

NAGK (N-acetyl-D-glucosamine kinase) belongs to the eukaryotic-type N-acetylglucosamine kinase family. NAGK converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. It is a prominent salvage enzyme of amino sugar metabolism in mammals. NAGK has been shown to interact with STK16 and LNX1. Also it has ManNAc kinase activity. Recombinant human NAGK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol NAGK
Entrez

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