Myotubularin Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Myotubularin Antibody [NBP1-86113] - Staining of human cell line U-251 MG shows localization to plasma membrane.

Immunohistochemistry-Paraffin: Myotubularin Antibody [NBP1-86113] - Staining of human testis shows moderate to strong cytoplasmic positivity in subsets of cells in seminiferous ducts.

Immunohistochemistry-Paraffin: Myotubularin Antibody [NBP1-86113] - Staining of human fallopian tube shows moderate to strong membranous positivity in subsets of glandular cells.

Immunohistochemistry-Paraffin: Myotubularin Antibody [NBP1-86113] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: Myotubularin Antibody [NBP1-86113] - Staining of human small intestine shows moderate to strong membranous positivity in glandular cells.

• Reactivity: Hu, Mu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Myotubularin Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: KFNVDGWTVYNPVEEYRRQGLPNHHWRITFINKCYELCDTYPALLVVPYRASDDDLRRVATFRSRNRIPVLSWIHPENKTVIVRCSQPLVGMSGKRNKDDEKYLDVIRETNKQISKLTIYDARPSVNAVANK
• Predicted Species: Mouse (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MTM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Myotubularin Protein (NBP1-86113PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (84%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Myotubularin Antibody - BSA Free

• CG2
• CNM
• EC 3.1.3.48
• MTMX
• myotubular myopathy 1
• myotubularin 1
• myotubularin
• XLMTM

Background

MTM1 encodes a dual-specificity phosphatase that acts on both phosphotyrosine and phosphoserine. It is required for muscle cell differentiation and mutations in this gene have been identified as being responsible for X-linked myotubular myopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myotubularin Antibody (NBP1-86113)(2)

Publications using NBP1-86113

• Agrawal PB, Pierson CR, Joshi M et al. SPEG Interacts with Myotubularin, and Its Deficiency Causes Centronuclear Myopathy with Dilated Cardiomyopathy. Am J Hum Genet 2014-08-07 [PMID: 25087613] (ICC/IF, Human)
• Gupta VA, Hnia K, Smith LL et al. Loss of Catalytically Inactive Lipid Phosphatase Myotubularin-related Protein 12 Impairs Myotubularin Stability and Promotes Centronuclear Myopathy in Zebrafish. PLoS Genet 2013-06-01 [PMID: 23818870]

Bioinformatics

• Gene Symbol: MTM1