EHM2 Antibody

Immunohistochemistry-Paraffin: EHM2 Antibody [NBP1-00181] - (3.75ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

EHM2 Antibody Summary

• Description: Novus Biologicals Goat EHM2 Antibody (NBP1-00181) is a polyclonal antibody validated for use in IHC and ELISA. Anti-EHM2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HNYQPQYHPNIHPSQ corresponding to internal region according to NP_060894.2, NP_061987.3.
• Specificity: This antibody is expected to recognise both reported isoforms (NP_060894.2; NP_061987.3).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EPB41L4B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:64000
• Application Notes: WB: Preliminary experiments gave an approx. 48 kDa band in human brain (hippocampus, Cerebellar cortex, cerebellum) and human bone marrow lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.6 kDa band according to NP_060894.2. The 48 kDa band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EHM2 Antibody

• band 4.1-like protein 4B
• CG1
• DKFZp761N1814
• EHM2FLJ21596
• erythrocyte membrane protein band 4.1 like 4B
• FERM-containing protein CG1
• Protein EHM2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EHM2 Antibody (NBP1-00181)(1)

Publication using NBP1-00181

• Diakowski W, Grzybek M, Sikorski AF. Protein 4.1, a component of the erythrocyte membrane skeleton and its related homologue proteins forming the protein 4.1/FERM superfamily. Folia Histochem Cytobiol 2006-01-01 [PMID: 17219717]

Bioinformatics

• Gene Symbol: EPB41L4B
• Entrez:
  • 54566(Human)
• Uniprot:
  • NP_060894.2(Human)