Myotrophin Antibody (1F3) - Azide and BSA Free

Western Blot: Myotrophin Antibody (1F3) [H00136319-M14] - Analysis of MTPN expression in transfected 293T cell line by MTPN monoclonal antibody (M14), clone 1F3. Lane 1: MTPN transfected lysatE (12.9 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Myotrophin Antibody (1F3) [H00136319-M14] - Analysis of monoclonal antibody to MTPN on HeLa cell. Antibody concentration 10 ug/ml

Sandwich ELISA: Myotrophin Antibody (1F3) [H00136319-M14] - Detection limit for recombinant GST tagged MTPN is 3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1F3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Myotrophin Antibody (1F3) - Azide and BSA Free Summary

• Immunogen: MTPN (AAH28093, 1 a.a. ~ 118 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MCDKEFMWALKNGGLDEVKDYVAKGEDVNRTLEGGRKPLHYAADCGQLEILEFLLLKGADINAPDKHHITPLLSAVYEGHVSCVKLLLSKGADKTVKGPDGLTAFEATDNQAIKALLQ
• Specificity: Reacts with myotrophin.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MTPN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: This antibody is reactive against transfected lysate for western blot and recombinant protein in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Myotrophin Antibody (1F3) - Azide and BSA Free

• FLJ31098
• FLJ99857
• GCDP
• granule cell differentiation protein
• MYOTROPHIN
• Protein V-1
• V-1

Background

The transcript produced from this gene is bi-cistronic and can encode both myotrophin and leucine zipper protein 6. The myotrophin protein is associated with cardiac hypertrophy, where it is involved in the conversion of NFkappa B p50-p65 heterodimers to p50-p50 and p65-p65 homodimers. This protein also has a potential function in cerebellar morphogenesis, and it may be involved in the differentiation of cerebellar neurons, particularly of granule cells. A cryptic ORF at the 3' end of this transcript uses a novel internal ribosome entry site and a non-AUG translation initiation codon to produce leucine zipper protein 6, a 6.4 kDa tumor antigen that is associated with myeloproliferative disease. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MTPN
• Entrez:
  • 136319(Human)
• Uniprot:
  • AAH28093(Human)