Myotilin Overexpression Lysate

Images

 
Western Blot: Myotilin Overexpression Lysate (Adult Normal) [NBL1-13445] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Myotilin.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Myotilin Overexpression Lysate Summary

Description

Myotilin Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC202797

Accession#: NM_006790

Protein Tag: C-MYC/DDK
Gene
MYOT

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
55.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Myotilin Overexpression Lysate

  • 57 kDa cytoskeletal protein
  • LGMD1A
  • limb-girdle muscular dystrophy 1A (autosomal dominant)
  • Myofibrillar titin-like Ig domains protein
  • myotilin
  • titin immunoglobulin domain protein (myotilin)
  • Titin immunoglobulin domain protein
  • TTIDLGMD1

Background

This gene encodes a cystoskeletal protein which plays a significant role in the stability of thin filaments during muscle contraction. This protein binds F-actin, crosslinks actin filaments, and prevents latrunculin A-induced filament disassembly. Mutations in this gene have been associated with limb-girdle muscular dystrophy and myofibrillar myopathies. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Myotilin Lysate (NBL1-13445) (0)

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Bioinformatics

Gene Symbol MYOT