Independent Antibodies: Western Blot: Myosin Phosphatase Antibody [NB110-38889] - Whole cell lysate (1 mg/IP, 20% IP loaded) from HeLa cells. NB110-38889 used for IP (3 ug/mg lysate). MYPT1 was also ...read more
The immunogen recognized by this antibody maps to a region between residue 400 and 450 of human Myosin Phosphatase Target Subunit 1 (Protein Phosphatase 1, Regulatory Subunit 12A) using the numbering given in entry NP_002471.1
Immunogen affinity purified
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115.281 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
protein phosphatase 1, regulatory (inhibitor) subunit 12A
Protein phosphatase myosin-binding subunit
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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