Myosin Phosphatase Antibody

Western Blot: Myosin Phosphatase Antibody [NB110-38889] - Whole cell lysate (1 mg/IP, 20% IP loaded) from HeLa cells. NB110-38889 used for IP (3 ug/mg lysate). MYPT1 was also immunoprecipitated by other MYPT1 antibody, recognize downstream epitopes.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Myosin Phosphatase Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 400 and 450 of human Myosin Phosphatase Target Subunit 1 (Protein Phosphatase 1, Regulatory Subunit 12A) using the numbering given in entry NP_002471.1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP1R12A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:5000
  • Immunoprecipitation 2-5 ug/mg lysate
• Theoretical MW: 115.281 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Positive Control:

  Myosin Phosphatase Lysate (NBL1-14674)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Myosin Phosphatase Antibody

• MBSmyosin phosphatase, target subunit 1
• MGC133042
• Myosin phosphatase target subunit 1
• Myosin phosphatase-targeting subunit 1
• MYPT1protein phosphatase 1 regulatory subunit 12A
• protein phosphatase 1, regulatory (inhibitor) subunit 12A
• Protein phosphatase myosin-binding subunit

Background

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myosin Phosphatase Antibody (NB110-38889)(1)

Publication using NB110-38889

• Gilkes DM, Xiang L, Lee SJ et al. Hypoxia-inducible factors mediate coordinated RhoA-ROCK1 expression and signaling in breast cancer cells. Proc Natl Acad Sci U S A 2014 Jan 21 [PMID: 24324133] (WB, Human)

Review for Myosin Phosphatase Antibody (NB110-38889) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Daniele Gilkes WB Human 08/17/2012

Summary

• Application: Western Blot
• Sample Tested: MDA-MB-231 Cell Lysate
• Species: Human
• Comments: Could have used a higher dilution or less lysate. Worked Well.

Blocking

• Blocking Details: 5% Milk in PBS-T, 20min, RT

Primary Anitbody

• Dilution Ratio: 1:1000 in 5% Milk in PBS-T, O/N, 4C

Secondary Antibody

• Secondary Description: goat anti-rabbit IgG-HRP
• Secondary Manufacturer Cat#: GE Healthcare
• Secondary Concentration: 1:5000

Details

• Detection Notes: ECL Prime, 10 seconds

Comments

• Comments: Could have used a higher dilution or less lysate. Worked Well.

Bioinformatics

• Gene Symbol: PPP1R12A
• Entrez:
  • 4659(Human)
• Uniprot:
  • O14974(Human)