Myosin Light Chain 2 Antibody (SN67-09)

Western Blot: Myosin Light Chain 2 Antibody (SN67-09) [NBP2-67649] - Western blot analysis of Myosin Light Chain 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours

Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody (SN67-09) [NBP2-67649] - Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Myosin Light Chain 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: Myosin Light Chain 2 Antibody (SN67-09) [NBP2-67649] - Analysis of Myosin Light Chain 2 on different lysates using anti-Myosin Light Chain 2 antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse liver Lane 2: Mouse brain

Western Blot: Myosin Light Chain 2 Antibody (SN67-09) [NBP2-67649] - Analysis of Myosin Light Chain 2 on hybrid fish (crucian-carp) skin tissue lysate using anti-Myosin Light Chain 2 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody (SN67-09) [NBP2-67649] - Analysis of paraffin-embedded mouse heart tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody (SN67-09) [NBP2-67649] - Analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Myosin Light Chain 2 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Myosin Light Chain 2 Antibody (SN67-09) [NBP2-67649] - Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Myosin Light Chain 2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

Myosin Light Chain 2 Antibody (SN67-09) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Myosin Light Chain 2 aa 117-166 / 166. (SwissProt: P10916 Human; SwissProt: P51667 Mouse; SwissProt: P08733 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MYL2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Myosin Light Chain 2 Antibody (SN67-09)

• cardiac ventricular myosin light chain 2
• CMH10myosin light chain 2
• DKFZp779C0562
• MLC2
• MLC-2
• MLC-2v
• myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• myosin, light chain 2, regulatory, cardiac, slow
• myosin, light polypeptide 2, regulatory, cardiac, slow
• regulatory light chain of myosin
• RLC of myosin
• slow cardiac myosin regulatory light chain 2

Background

MYL2 (myosin, light chain 2, regulatory, cardiac, slow), also known as MLC-2, MLC2v. Entrez protein NP_000423. MYL2 associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. It is an hexamer of two heavy chains and four light chains that is predominantly expressed in adult cardiac ventricle muscle. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYL2