Myosin Light Chain 2 Antibody (EPR3741)

Product Discontinued

Myosin Light Chain 2 Antibody (EPR3741) Summary

• Immunogen: A synthetic peptide corresponding to residues in human Myosin Light Chain 2 was used as an immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MYL2
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-250
  • Immunoprecipitation 1:50
  • Western Blot 1:10000-50000
• Application Notes: Use in IHC-Fr reported in scientific literature (PMID:33268364).
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for Myosin Light Chain 2 Antibody (EPR3741)

• cardiac ventricular myosin light chain 2
• CMH10myosin light chain 2
• DKFZp779C0562
• MLC2
• MLC-2
• MLC-2v
• myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• myosin, light chain 2, regulatory, cardiac, slow
• myosin, light polypeptide 2, regulatory, cardiac, slow
• regulatory light chain of myosin
• RLC of myosin
• slow cardiac myosin regulatory light chain 2

Background

MYL2 (myosin, light chain 2, regulatory, cardiac, slow), also known as MLC-2, MLC2v. Entrez protein NP_000423. MYL2 associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. It is an hexamer of two heavy chains and four light chains that is predominantly expressed in adult cardiac ventricle muscle. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myosin Light Chain 2 Antibody (NBP1-40754)(2)

Publications using NBP1-40754

• Hatzistergos KE, Durante MA, Valasaki K et al. A novel cardiomyogenic role for Isl1+ neural crest cells in the inflow tract Science advances 2020-12-01 [PMID: 33268364] (IHC-Fr, Human)
• Kirk JA, Holewinski RJ, Kooij V et al. Cardiac resynchronization sensitizes the sarcomere to calcium by reactivating GSK-3beta. J Clin Invest 2013-12-02 [PMID: 24292707] (WB, Canine)

Review for Myosin Light Chain 2 Antibody (NBP1-40754) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer WB Other 12/23/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 24292707
• Species: Other

Bioinformatics

• Gene Symbol: MYL2
• Entrez:
  • 4633(Human)
  • 17906(Mouse)
  • 363925(Rat)
• Uniprot:
  • P10916(Human)