Myosin Heavy Chain 1 Antibody

Product Discontinued

Myosin Heavy Chain 1 Antibody Summary

• Immunogen: Bovine uterine myosin
• Specificity: NB100-64587 reacts with heavy chain myosin from smooth muscle or non-muscle sources. Does not cross react with Myosin Skeletal and Cardiac, Actin, Tropomyosin and Tubulin. In Western blotting major bands were observed at 190-200 kD. Fragments of heavy chain myosin may also be seen at 170 kD, 130 kD and 110 kD.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYH1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:25-1:100
  • Western Blot 1:100-1:2000
• Application Notes: Immunofluorescence and Western Blot Cells were fixed at ambient temperatures (37C) for 5-10 minutes in freshly prepared formaldehyde at a concentration of 2-4%, buffered with a balanced salt solution.

Reactivity Notes

Bovine

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Phosphate Buffered Saline
• Preservative: 0.02% Sodium Azide
• Purity: IgG purified

Alternate Names for Myosin Heavy Chain 1 Antibody

• DFNA4
• FLJ13881
• FLJ43092
• KIAA2034DKFZp667A1311
• MHC16
• MYH1
• MYH14 variant protein
• MYH17
• MyHC-2x
• MyHC-IIx/d
• Myosin Heavy Chain 1
• Myosin heavy chain 14
• Myosin heavy chain, non-muscle IIc
• myosin
• myosin, heavy chain 14
• myosin, heavy chain 14, non-muscle
• myosin, heavy polypeptide 14
• myosin-14
• NMHC II-C
• NMHC-II-C
• Non-muscle myosin heavy chain IIc
• nonmuscle myosin heavy chain II-C

Background

Myosin II is the so called "classical" myosin, being the first type of myosin to be discovered. Myosin II forms bipolar filaments that interact with actin filaments to produce contraction. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. The myosin II molecule is composed of two heavy chains and two sets of light chains. The light chains are the Essential Light Chains (ELC) and Regulatory Light Chains (RLC). Overall activity and polymerization can be modulated by phosphorylation on the light chains and the C terminus of the heavy chains respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYH1