Myosin 5a Antibody

Images

 
Western Blot: Myosin 5a Antibody [NBP1-49858] - Analysis of Myosin 5a in Jurkat lysate (35ug protein in RIPA buffer) using this antibody at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Myosin 5a Antibody [NBP1-49858] - (1ug/ml) staining of Mouse Skeletal Muscle (first panel, and in green in third panel). Alpha-bungaratoxin staining in middle panel and in red in ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

Myosin 5a Antibody Summary

Immunogen
Peptide with sequence C-ETKQLELDLN corresponding to internal region according to NP_001135967.1.
Epitope
ETKQLELDLN - internal region
Specificity
This antibody is expected to recognize both reported isoforms (NP_000250.3; NP_001135967.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MYO5A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
IF: Transverse sections of mouse Skeletal Muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodiesscientific literature (PMID: 20133847). WB: Approx. 200 kDa band observed in Jurkat lysates (calculated MW of 215 kDa band according to NP_000250.3).
Publications
Read Publications using NBP1-49858.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Myosin 5a Antibody

  • Dilute myosin heavy chain, non-muscle
  • GS1myosin, heavy polypeptide kinase
  • MYH12myosin-12
  • MYO5
  • Myosin heavy chain 12
  • myosin V
  • myosin VA (heavy chain 12, myoxin)
  • myosin VA (heavy polypeptide 12, myoxin)
  • Myosin-12
  • myosin-Va
  • myoxin
  • MYR12

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myosin 5a Antibody (NBP1-49858)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MYO5A
Entrez
Uniprot