Myosin 5a Antibody

Western Blot: Myosin 5a Antibody [NBP1-49858] - Analysis of Myosin 5a in Jurkat lysate (35ug protein in RIPA buffer) using this antibody at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: Myosin 5a Antibody [NBP1-49858] - (1ug/ml) staining of Mouse Skeletal Muscle (first panel, and in green in third panel). Alpha-bungaratoxin staining in middle panel and in red in third panel. Detected by Fluorescence. Data kindly provided by Dr. Rudiger Rudolf, Karlsruhe, Germany

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Myosin 5a Antibody Summary

• Description: Novus Biologicals Goat Myosin 5a Antibody (NBP1-49858) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-Myosin 5a Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-ETKQLELDLN corresponding to internal region according to NP_001135967.1.
• Epitope: ETKQLELDLN - internal region
• Specificity: This antibody is expected to recognize both reported isoforms (NP_000250.3; NP_001135967.1).
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MYO5A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 3 ug/ml
• Application Notes: IF: Transverse sections of mouse Skeletal Muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodiesscientific literature (PMID: 20133847). WB: Approx. 200 kDa band observed in Jurkat lysates (calculated MW of 215 kDa band according to NP_000250.3).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Myosin 5a Antibody

• Dilute myosin heavy chain, non-muscle
• GS1myosin, heavy polypeptide kinase
• MYH12myosin-12
• MYO5
• Myosin heavy chain 12
• myosin V
• myosin VA (heavy chain 12, myoxin)
• myosin VA (heavy polypeptide 12, myoxin)
• Myosin-12
• myosin-Va
• myoxin
• MYR12

Background

The MYO5 gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The p

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myosin 5a Antibody (NBP1-49858)(2)

Publications using NBP1-49858

• Roder IV, Choi KR, Reischl M et al. Myosin Va cooperates with PKA RIalpha to mediate maintenance of the endplate in vivo. Proc Natl Acad Sci USA 2010-02-01 [PMID: 20133847]
• Guey LT, Garcia-Closas M, Murta-Nascimento C et al. Genetic susceptibility to distinct bladder cancer subphenotypes. Eur Urol 2010-02-01 [PMID: 19692168]

Bioinformatics

• Gene Symbol: MYO5A
• Entrez:
  • 4644(Human)
• Uniprot:
  • NP_000250.3(Human)