MyoD Antibody (474515) [Unconjugated]

MyoD was detected in fixed C2C12 mouse myoblast cell line (Positive) and absent in RD cells (Negative) using Mouse Anti-Human MyoD Monoclonal Antibody (Catalog # MAB5966) at 25 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Cdkn1c is required for the proper formation of iBAT.(A) Photograph of E18.5 WT and Cdkn1c-/+ (KOMAT) fetuses with position of iBAT depot highlighted by dotted black line. (B) H&E staining of iBAT sections of E18.5 WT and KOMAT iBAT. (C) QPCR of Cdkn1c and the adipocyte regulators Rb1, PPAR gamma , C/EBP alpha and C/EBP beta , pan-adipocyte genes Fabp4, Cidec and Plin1, BAT-selective genes Ppargc1a, Cidea, Prdm16, Ucp1 and Elovl3, mitochondrial genes Cycs and Cox2, and the skeletal muscle-selective genes Myf5 and Myod1 in E18.5 KOMAT iBAT relative to WT (n = 4 per genotype). (D) Mitochondrial DNA content of iBAT from E18.5 KOMAT iBAT relative to WT (n = 6 per genotype). (E) Western blot analysis of UCP1 and beta -ACTIN in E18.5 iBAT isolated from two WT and two KOMAT fetuses. Within litter comparison. (F) Western blot analysis of CDKN1C, PRDM16 and beta -ACTIN in E18.5 iBAT isolated from two WT and two KOMAT fetuses. Within litter comparison. (G) Western blot analysis of MYOD and GAPDH in E18.5 E18.5 iBAT isolated from two WT and two KOMAT fetuses. Within litter comparison. Data expressed as mean ± SEM, t test. * P <0.05; ** P <0.01; *** P <0.005. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26963625), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 474515
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MyoD Antibody (474515) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human MyoD
  Glu77-Gly215
  Accession # P15172
• Specificity: Detects human MyoD in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYOD1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MyoD Antibody (474515) [Unconjugated]

• bHLHc1
• bHLHc1BHLHC1
• Class C basic helix-loop-helix protein 1
• MYF3
• MYF3Myf-3
• MyoD
• MYOD1
• MYODmyoblast determination protein 1
• myogenic differentiation 1
• Myogenic factor 3myf-3
• PUM

Background

MyoD (myoblast determination protein 1), also called Myf-3 (myogenic factor 3) is an ~44 kDa nuclear protein in the MyoD family of muscle-specific bHLH transcription factors. MyoD family members heterodimerize with E proteins and cooperate with MEF2 family transcription factors to regulate expression of skeletal muscle-specific genes. MyoD is essential for skeletal muscle differentiation. Acetylation at lysines 99, 102 and 104 further regulates its activity. Human MyoD shares 96% amino acid identity with mouse and rat MyoD over the sequence used as the immunogen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MyoD Antibody (MAB5966)(2)

Publications using MAB5966

• R Mikši?nas, S Labeit, D Bironait? The Effect of Heat Shock on Myogenic Differentiation of Human Skeletal-Muscle-Derived Mesenchymal Stem/Stromal Cells Cells, 2022-10-13;11(20):. 2022-10-13 [PMID: 36291076] (Flow Cytometry, Human)
• R Yang, X Yang, S Liu, L Ming, Z Zhou, Y Liang, Y Zhao, F Zhou, ZH Deng, Y Jin Activation of the Wnt/ β-catenin pathway by an inflammatory microenvironment affects the myogenic differentiation capacity of human laryngeal mucosa mesenchymal stromal cells Stem Cells Dev., 2018-05-09;0(0):. 2018-05-09 [PMID: 29644939] (IHC-P, Xenograft)

Bioinformatics

• Gene Symbol: MYOD1
• Uniprot:
  • P15172()