MYO18A Antibody - BSA Free

Immunoprecipitation: MYO18A Antibody [NB100-79839] - Detection of Human Myo18A/Myosin XVIIIA by Western Blot of Immunoprecipitates. Samples: RIPA whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Myo18A/Myosin XVIIIA antibody NB100-79839 used for IP at 3 mcg/mg lysate. Myo18A/Myosin XVIIIA was also immunoprecipitated by rabbit anti- Myo18A/Myosin XVIIIA antibody NB100-79840, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 1 second.

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MYO18A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MYO18A Antibody - BSA Free (NB100-79839) is a polyclonal antibody validated for use in IP. Anti-MYO18A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen this antibody was made to, maps to a region between residue 300 and 350 of human myosin 18A using the numbering given in entry NP_510880.2 (GeneID 399687).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYO18A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 mcg/mg lysate
• Application Notes: This antibody has not performed satisfactorily when used for WB of Myo18A/Myosin XVIIIA in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Myo18A/Myosin XVIIIA.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MYO18A Antibody - BSA Free

• KIAA0216DKFZp686L0243
• MAJN
• Molecule associated with JAK3 N-terminus
• myosin 18A
• Myosin containing a PDZ domain
• myosin containing PDZ domain
• myosin XVIIIA
• myosin-XVIIIa
• MYSPDZ
• SP-A receptor subunit SP-R210 alphaS
• SPR210

Background

Myo18A/Myosin XVIIIA is an unconventional myosin that contains a lysine and glutamine (KE)-rich domain and a PDZ domain. The KE and PDZ domains appear to direct subcellular localization of the Myo18A variants. It is a dimeric actin binding protein that may play a role in the stabilization and organization of the actin cytoskeleton. Recently a cell surface form of Myo18A has been identified as the receptor for surfactant protein A, a lipid-associated lung collectin that mediates innate and adaptive immune responses in the lungs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MYO18A Antibody (NB100-79839)(1)

Publication using NB100-79839

• Aguilar-Cuenca R, Llorente-GonzAlez C, Chapman JR et al. Tyrosine Phosphorylation of the Myosin Regulatory Light Chain Controls Non-muscle Myosin II Assembly and Function in Migrating Cells Curr. Biol. 2020-06-02 [PMID: 32502416]

Bioinformatics

• Gene Symbol: MYO18A
• Entrez:
  • 399687(Human)
• Uniprot:
  • NP_510880.2(Human)