MRCK Antibody

Immunohistochemistry-Paraffin: MRCK Antibody [NB300-829] - Staining of paraffin embedded Human Cerebellum. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: MRCK Antibody [NB300-829] - Staining of paraffin embedded Human Small Intestine. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MRCK Antibody Summary

• Description: Novus Biologicals Goat MRCK Antibody (NB300-829) is a polyclonal antibody validated for use in IHC and ELISA. Anti-MRCK Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence SGEVRLRQLEQFI-C corresponding to N-Terminus according to NP_003598.2.
• Epitope: SGEVRLRQLEQFI-C
• Specificity: This antibody is expected to recognise both reported Human isoforms (NP_055641.3 and NP_003598.2).
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CDC42BPA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.75 ug/mL
  • Immunohistochemistry-Paraffin 3.75 ug/mL
  • Peptide ELISA Detection limit 1:16000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MRCK Antibody

• CDC42 binding protein kinase alpha (DMPK-like)
• CDC42 binidng protein kinase beta
• CDC42-binding protein kinase alpha (DMPK-like)
• CDC42-binding protein kinase alpha
• DMPK-like alpha
• EC 2.7.11
• EC 2.7.11.1
• KIAA0451DKFZp686L1738
• MRCK alpha
• MRCK
• MRCKADKFZp686P1738
• Myotonic dystrophy kinase-related CDC42-binding kinase alpha
• myotonic dystrophy kinase-related CDC42-binding protein kinase alpha
• Myotonic dystrophy protein kinase-like alpha
• PK428FLJ23347
• serine/threonine-protein kinase MRCK alpha
• ser-thr protein kinase PK428
• ser-thr protein kinase related to the myotonic dystrophy protein kinase

Background

The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MRCK Antibody (NB300-829)(1)

Publication using NB300-829

• Zhao Y, Loyer P, Li H et al. Cloning and chromosomal location of a novel member of the myotonic dystrophy family of protein kinases. J Biol Chem 1997-04-11 [PMID: 9092543]

Bioinformatics

• Gene Symbol: CDC42BPA
• Entrez:
  • 8476(Human)
  • 226751(Mouse)
  • 114116(Rat)
• Uniprot:
  • NP_055641(Human)