MYL9 Overexpression Lysate (Native)

Images

 
Western Blot: MYL9 Overexpression Lysate (Adult Normal) [NBL1-13430] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MYL9.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MYL9 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201080 and based on accession number NM_181526. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens myosin, light chain 9, regulatory (MYL9), transcript variant 2, mRNA.
Gene
MYL9

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
13.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MYL9

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MYL9 Overexpression Lysate (Native)

  • LC2020 kDa myosin light chain
  • MLC2Myosin RLC
  • MRLC1MLC-2C
  • myosin regulatory light chain 1
  • Myosin regulatory light chain 2, smooth muscle isoform
  • Myosin regulatory light chain 9
  • Myosin regulatory light chain MRLC1
  • myosin regulatory light polypeptide 9
  • myosin, light chain 9, regulatory
  • myosin, light polypeptide 9, regulatory
  • MYRL2MGC3505

Background

Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1, resulting in a shorter isoform (b) compared to isoform a.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol MYL9