Human MYL9 ELISA Kit (Colorimetric)

ELISA: Human MYL9 ELISA Kit (Colorimetric) [NBP3-39559] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Standard Curve Range: 78.125 - 5000 pg/mL (example only; lot dependent)
• Sensitivity: 29 pg/mL (example only; lot dependent)

Human MYL9 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 78.125 - 5000 pg/mL (example only; lot dependent)
• Sensitivity: 29 pg/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MYL9

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human MYL9 ELISA Kit (Colorimetric)

• LC2020 kDa myosin light chain
• MLC2Myosin RLC
• MRLC1MLC-2C
• myosin regulatory light chain 1
• Myosin regulatory light chain 2, smooth muscle isoform
• Myosin regulatory light chain 9
• Myosin regulatory light chain MRLC1
• myosin regulatory light polypeptide 9
• myosin, light chain 9, regulatory
• myosin, light polypeptide 9, regulatory
• MYRL2MGC3505

Background

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/ cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human, mouse and rat have almost identical sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: MYL9