Recombinant Human MYL5 His Protein

SDS-Page: Recombinant Human MYL5 Protein [NBP1-72469] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human MYL5 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-173 of Human MYL5
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMASRKT KKKEGGALRA QRASSNVFSN FEQTQIQEFK EAFTLMDQNR DGFIDKEDLK DTYASLGKTN VKDDELDAML KEASGPINFT MFLNLFGEKL SGTDAEETIL NAFKMLDPDG KGKINKEYIK RLLMSQADKM TAEEVDQMFQ FASIDVAGNL DYKALSYVIT HGEEKEE

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MYL5
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 22.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1 M NaCl, 1 mM DTT, 0.1 mM PMSF
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human MYL5 His Protein

• light polypeptide 5, regulatory
• MYLC2
• MyLC-2
• myosin light chain 5
• Myosin regulatory light chain 5
• myosin, light chain 5, regulatory
• Superfast myosin regulatory light chain 2

Background

Myosin regulatory light chain 5, also known as MYL5, is a hexameric ATPase cellular motor protein. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL5 is a regulatory light chain and is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia. Reconstitution of myosin with regulatory light chain 5 or alkali light chain increases filament velocity to intermediate rates, and readdition of both classes of light chains fully restores the original sliding velocity. Recombinant human MYL5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MYL5