MYH7 Antibody (2021A) [Unconjugated]

Western blot shows lysates of human heart (ventricle) tissue. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human MYH7 Monoclonal Antibody (Catalog # MAB90961) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MYH7 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MYH7 was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Rabbit Anti-Human MYH7 Monoclonal Antibody (Catalog # MAB90961) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 2021A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

MYH7 Antibody (2021A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human MYH7 synthetic peptide
  Accession # P12883
• Specificity: Detects human MYH7 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MYH7 Antibody (2021A) [Unconjugated]

• cardiac muscle beta isoform
• CMD1S
• CMD1SMGC138376
• CMH1
• DKFZp451F047
• MGC138378
• MPD1
• MYH7
• MYHCB
• MyHC-beta
• myhc-slow
• myopathy, distal 1
• myosin heavy chain (AA 1-96)
• myosin, heavy chain 7, cardiac muscle, beta
• myosin, heavy polypeptide 7, cardiac muscle, beta
• Myosin-7
• rhabdomyosarcoma antigen MU-RMS-40.7A
• SPMD
• SPMM

Background

MYH7 encodes the beta myosin heavy chain (MHC-beta ) which is a component of cardiac muscle myosin mainly expressed in the ventricle of fetal heart and represents the minority myosin in the adult heart. This is the 'slow form' of cardiac myosin as opposed to the 'fast form' (MYH6, aka MHC-alpha ) expressed more predominantely in the atria of the fetal heart and is the predominant myosin in the adult heart. The two isoforms of cardiac MHC alpha  and  beta  display 93% homology but have significantly different enzymatic properties, with  alpha  having 150-300% the contractile velocity and 60-70% actin attachment time as that of beta . Several mutations in MYH7 have been associated with inherited cardiomyopathies paraspinal and proximal muscle atrophy. MYH7 is a 223 kDa protein composed of 1935 amino acids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MYH7 Antibody (MAB90961)(3)

Publications using MAB90961

• Abraham, E;Kostina, A;Volmert, B;Roule, T;Huang, L;Yu, J;Williams, AE;Megill, E;Douglas, A;Pericak, OM;Morris, A;Stronati, E;Larrinaga-Zamanillo, A;Fueyo, R;Zubillaga, M;Andrake, MD;Akizu, N;Aguirre, A;Estaras, C; A retinoic acid:YAP1 signaling axis controls atrial lineage commitment Cell reports 2025-05-07 [PMID: 40343798] (Immunocytochemistry, Human)
• Zhen, J;Sheng, X;Chen, T;Yu, H; Histone acetyltransferase Kat2a regulates ferroptosis via enhancing Tfrc and Hmox1 expression in diabetic cardiomyopathy Cell death & disease 2024-06-10 [PMID: 38858351] (Western Blot, Mouse)
• Y Zhao, C Wang, C Wang, X Hong, J Miao, Y Liao, L Zhou, Y Liu An essential role for Wnt/?-catenin signaling in mediating hypertensive heart disease Sci Rep, 2018-06-12;8(1):8996. 2018-06-12 [PMID: 29895976] (Western Blot, Rat)

Bioinformatics

• Uniprot:
  • P12883()