Myeloid leukemia factor 1 Overexpression Lysate (Native)

Images

 
Western Blot: Myeloid leukemia factor 1 Overexpression Lysate (Adult Normal) [NBL1-13132] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Myeloid leukemia factor ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Myeloid leukemia factor 1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202774 and based on accession number NM_022443. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 1, mRNA.
Gene
MLF1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
30.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Myeloid leukemia factor 1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Myeloid leukemia factor 1 Overexpression Lysate (Native)

  • Myelodysplasia-myeloid leukemia factor 1
  • myeloid leukemia factor 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB110-61646
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-27202
Species: Hu, Mu, Rt, Po, Ca, Ch, ChHa, Fe, Op, Other, Pm
Applications: WB, Simple Western
NBP1-85435
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-03726
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-77938
Species: Hu, Bv, Ca, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-89827
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IF
NB100-55256
Species: Hu
Applications: WB, IHC-P, IP
NBP1-87769
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
H00171023-M05
Species: Hu
Applications: WB, ELISA
NBP1-47883
Species: Hu, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
H00064326-M01
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF
NBP1-85436
Species: Hu
Applications: IHC, IHC-P
MAB1259
Species: Hu
Applications: CyTOF-reported, ICC, ICFlow
NBP2-46383
Species: Hu
Applications: WB, Flow, IHC
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
28100002
Species: Hu
Applications: WB, ELISA, IHC, IHC-P

Publications for Myeloid leukemia factor 1 Lysate (NBL1-13132) (0)

There are no publications for Myeloid leukemia factor 1 Lysate (NBL1-13132).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Myeloid leukemia factor 1 Lysate (NBL1-13132) (0)

There are no reviews for Myeloid leukemia factor 1 Lysate (NBL1-13132). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Myeloid leukemia factor 1 Lysate (NBL1-13132) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Myeloid leukemia factor 1 Products

Bioinformatics Tool for Myeloid leukemia factor 1 Lysate (NBL1-13132)

Discover related pathways, diseases and genes to Myeloid leukemia factor 1 Lysate (NBL1-13132). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Myeloid leukemia factor 1 Lysate (NBL1-13132)

Discover more about diseases related to Myeloid leukemia factor 1 Lysate (NBL1-13132).
 

Pathways for Myeloid leukemia factor 1 Lysate (NBL1-13132)

View related products by pathway.

PTMs for Myeloid leukemia factor 1 Lysate (NBL1-13132)

Learn more about PTMs related to Myeloid leukemia factor 1 Lysate (NBL1-13132).
 

Research Areas for Myeloid leukemia factor 1 Lysate (NBL1-13132)

Find related products by research area.

Blogs on Myeloid leukemia factor 1

There are no specific blogs for Myeloid leukemia factor 1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Myeloid leukemia factor 1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol MLF1