MxA/Mx1 Antibody (HL2051) - Azide and BSA Free

Immunohistochemistry-Paraffin: MxA/Mx1 Antibody (HL2051) - Azide and BSA Free [NBP3-25572] - MX1 antibody [HL2051] detects MX1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. MX1 stained by MX1 antibody [HL2051] (NBP3-25572) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: MxA/Mx1 Antibody (HL2051) - Azide and BSA Free [NBP3-25572] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MX1 antibody [HL2051] (NBP3-25572) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemistry-Paraffin: MxA/Mx1 Antibody (HL2051) - Azide and BSA Free [NBP3-25572] - MX1 antibody [HL2051] detects MX1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. MX1 stained by MX1 antibody [HL2051] (NBP3-25572) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: MxA/Mx1 Antibody (HL2051) - Azide and BSA Free [NBP3-25572] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MX1 antibody [HL2051] (NBP3-25572) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: MxA/Mx1 Antibody (HL2051) - Azide and BSA Free [NBP3-25572] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MX1 antibody [HL2051] (NBP3-25572) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: MxA/Mx1 Antibody (HL2051) - Azide and BSA Free [NBP3-25572] - MX1 antibody [HL2051] detects MX1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. MX1 stained by MX1 antibody [HL2051] (NBP3-25572) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

MX1 antibody [HL2051] detects MX1 protein by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MX1 stained by MX1 antibody [HL2051] diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI.

• Reactivity: Hu, Mu, Ca, Fe
• Applications: WB, ICC/IF, IHC
• Clone: HL2051
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MxA/Mx1 Antibody (HL2051) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit MxA/Mx1 Antibody (HL2051) - Azide and BSA Free (NBP3-25572) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human MxA/Mx1
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MX1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 76 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for MxA/Mx1 Antibody (HL2051) - Azide and BSA Free

• human interferon-regulated resistance GTP-binding protein MXA10IFI-78Khomolog of murine (interferon-inducibleprotein p78)
• IFI78
• IFI-78K
• Mx1
• MxA
• myxovirus (influenza virus) resistance 1, interferon-inducible protein p78(mouse)

Background

In mouse, the interferon-inducible Mx protein is responsible for a specific antiviral state against influenza virus infection. The protein encoded by this gene is similar to the mouse protein as determined by its antigenic relatedness, induction conditions, physicochemical properties, and amino acid analysis. This cytoplasmic protein is a member of both the dynamin family and the family of large GTPases. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MX1