MURF3 Antibody

Western Blot: MURF3 Antibody [NB100-1197] - (1ug/ml) of human muscle lysate (RIPA buffer, 35ug total

Immunohistochemistry-Paraffin: MURF3 Antibody [NB100-1197] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MURF3 Antibody Summary

• Description: Novus Biologicals Goat MURF3 Antibody (NB100-1197) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-MURF3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-GSAGPEEERPDGP corresponding to C-Terminus according to NP_115935.3, NP_912730.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRIM54
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Experiments gave bands at approx. 45 kDa band and 38 kDa band in human skeletal muscle lysate after 1 ug/ml antibody staining. These bands correspond to the reported isoforms with calculated MWs of 43.1 kDa band according to NP_115935, and 38.6 kDa band according to NP_912730.
  IHC: Paraffin embedded Human Skeletal Muscle.
• Control:

  MURF3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MURF3 Antibody

• MuRF
• muRF3
• MURF-3
• MURFtripartite motif-containing protein 54
• Muscle-specific RING finger protein 3
• Muscle-specific RING finger protein
• RING finger protein 30muscle-specific RING-finger protein 3
• RNF30
• tripartite motif containing 54
• tripartite motif-containing 54

Background

The protein encoded by this gene contains a RING finger motif and is highly similar to the ring finger proteins RNF28/MURF1 and RNF29/MURF2. In vitro studies demonstrated that this protein, RNF28, and RNF29 form heterodimers, which may be important for the regulation of titin kinase and microtubule-dependent signal pathways in striated muscles. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MURF3 Antibody (NB100-1197)(1)

Publication using NB100-1197

• Centner T, Yano J, Kimura E et al. Identification of muscle specific ring finger proteins as potential regulators of the titin kinase domain. J Mol Biol 2001-03-02 [PMID: 11243782]

Bioinformatics

• Gene Symbol: TRIM54
• Entrez:
  • 57159(Human)
• Uniprot:
  • NP_115935.(Human)