MURF3 Antibody

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Western Blot: MURF3 Antibody [NB100-1197] - (1ug/ml) of human muscle lysate (RIPA buffer, 35ug total
Immunohistochemistry-Paraffin: MURF3 Antibody [NB100-1197] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MURF3 Antibody Summary

Immunogen
Peptide with sequence C-GSAGPEEERPDGP corresponding to C-Terminus according to NP_115935.3, NP_912730.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TRIM54
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Experiments gave bands at approx. 45 kDa band and 38 kDa band in human skeletal muscle lysate after 1 ug/ml antibody staining. These bands correspond to the reported isoforms with calculated MWs of 43.1 kDa band according to NP_115935, and 38.6 kDa band according to NP_912730.
IHC: Paraffin embedded Human Skeletal Muscle.
Control
MURF3 Overexpression Lysate
Publications
Read Publication using NB100-1197.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MURF3 Antibody

  • MuRF
  • muRF3
  • MURF-3
  • MURFtripartite motif-containing protein 54
  • Muscle-specific RING finger protein 3
  • Muscle-specific RING finger protein
  • RING finger protein 30muscle-specific RING-finger protein 3
  • RNF30
  • tripartite motif containing 54
  • tripartite motif-containing 54

Background

The protein encoded by this gene contains a RING finger motif and is highly similar to the ring finger proteins RNF28/MURF1 and RNF29/MURF2. In vitro studies demonstrated that this protein, RNF28, and RNF29 form heterodimers, which may be important for the regulation of titin kinase and microtubule-dependent signal pathways in striated muscles. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MURF3 Antibody (NB100-1197)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol TRIM54
Entrez
Uniprot