MuRF1/TRIM63 Antibody (6G6)

Images

 
Western Blot: MuRF1/TRIM63 Antibody (6G6) [H00084676-M01] - Analysis of TRIM63 expression in transfected 293T cell line by TRIM63 monoclonal antibody (M01), clone 6G6. Lane 1: TRIM63 transfected lysate(40.2 KDa). Lane ...read more
Immunocytochemistry/ Immunofluorescence: MuRF1/TRIM63 Antibody (6G6) [H00084676-M01] - Analysis of monoclonal antibody to TRIM63 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: MuRF1/TRIM63 Antibody (6G6) [H00084676-M01] - Analysis of TRIM63 over-expressed 293 cell line, cotransfected with TRIM63 Validated Chimera RNAi ( Cat # H00084676-R01V ) (Lane 2) or non-transfected control ...read more
Sandwich ELISA: MuRF1/TRIM63 Antibody (6G6) [H00084676-M01] - Detection limit for recombinant GST tagged TRIM63 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
6G6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

MuRF1/TRIM63 Antibody (6G6) Summary

Immunogen
TRIM63 (NP_115977, 254 a.a. ~ 353 a.a) partial recombinant protein with GST tag.DKSTKLVETAIQSLDEPGGATFLLTAKQLIKSIVEASKGCQLGKTEQGFENMDFFTLDLEHIADALRAIDFGTDEEEEEFIEEEDQEEEESTEGKEEGH
Specificity
TRIM63 - tripartite motif-containing 63
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TRIM63
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA and immunofluorescence.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MuRF1/TRIM63 Antibody (6G6)

  • EC 6.3.2.-
  • IRF
  • IRFMURF2
  • Iris RING finger protein
  • MuRF1
  • MuRF-1
  • muscle specific ring finger protein 2
  • Muscle-specific RING finger protein 1
  • RING finger protein 28FLJ32380
  • RNF28
  • RNF28E3 ubiquitin-protein ligase TRIM63
  • SMRZ
  • SMRZMURF-1
  • Striated muscle RING zinc finger protein
  • TRIM63
  • tripartite motif containing 63
  • tripartite motif-containing 63
  • Tripartite motif-containing protein 63

Background

This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TRIM63
Entrez
OMIM
Uniprot