Human MUC5B ELISA Kit (Colorimetric)

ELISA: Human MUC5B ELISA Kit (Colorimetric) [NBP2-76705] - Samples were spiked with high concentrations of Human MUC5B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human MUC5B ELISA Kit (Colorimetric) [NBP2-76705] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.625 - 40ng/mL
• Sensitivity: 0.375ng/mL

Human MUC5B ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 0.625 - 40ng/mL
• Sensitivity: 0.375ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.17
• Intra-Assay: CV% < 5.15
• Spike Recovery: 86-108%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MUC5B

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human MUC5B ELISA Kit (Colorimetric)

• high molecular weight salivary mucin MG1
• mucin 5, subtype B, tracheobronchial
• mucin 5B, oligomeric mucus/gel-forming
• sublingual gland mucin
• tracheobronchial

Background

MUC5B, also known as Mucin 5B, is a long 5762 amino acid protein that is 596 kDa, expressed on surface airway epithelia, it is a major contributor to the lubricating and viscoelastic properties of whole saliva, normal lung mucus and cervical mucus. Disease research is being performed in relation to MUC5B and chronic obstructive pulmonary disease, cervicitis, sinusitis, polyposis, pseudomyxoma peritonei, otitis media, dry eye syndrome, ampulla of vater carcinoma, common cold, copd, dental caries, diffuse panbronchiolitis, biliary papillomatosis, panbronchiolitis, atrophic gastritis, Barrett's esophagus, cystic fibrosis lung disease, peptic ulcer, bronchitis, gastritis, sinusitis, asthma, laryngitis, and nasopharyngitis. This protein has been shown to have interactions with AMY1A, AMY1B, AMY1C, HTN1, STATH, and over 40 other proteins in Addition of GalNAc to the Tn antigen via an alpha-1,6 linkage forms a Core 7 glycoprotein, O-linked glycosylation of mucins, Metabolism of proteins, Post-translational protein modification, Addition of galactose to the Tn antigen via an alpha-1,3 linkage forms a Core 8 glycoprotein, Addition of GlcNAc to the Tn antigen via a beta-1,6 linkage forms a Core 6 glycoprotein, Termination of O-glycan biosynthesis, Salivary secretion, Mucin expression in CF via TLRs, EGFR signaling pathways, Mucin expression in CF via IL-6, IL-17 signaling pathways, and Selected targets of CREB1 pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for MUC5B ELISA Kit (NBP2-76705)(1)

Publication using NBP2-76705

• Lin Weng, Wei Liu, Lingye Wang, Zhichao Wu, Dehao Liu, Yihua Lin, Shuli Song, Ciyuan Yu, Yaqiong Chen, Juan Chen, Shengxiang Ge Serum MUC5AC protein levels are correlated with the development and severity of connective tissue disease-associated pulmonary interstitial lesions Frontiers in Immunology 2022-09-15 [PMID: 36189284]

Bioinformatics

• Gene Symbol: MUC5B