mu Crystallin Antibody (OTI1G7) - Azide and BSA Free

Western Blot: mu Crystallin Antibody (OTI1G7) - Azide and BSA Free [NBP2-72825] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mu Crystallin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mu Cryst

Immunohistochemistry: mu Crystallin Antibody (OTI1G7) - Azide and BSA Free [NBP2-72825] - Staining of paraffin-embedded Human liver tissue using anti-mu Crystallin mouse monoclonal antibody.

Flow Cytometry: mu Crystallin Antibody (OTI1G7) - Azide and BSA Free [NBP2-72825] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY mu Crystallin.

Western Blot: mu Crystallin Antibody (OTI1G7) - Azide and BSA Free [NBP2-72825] - Analysis of extracts (10ug) from 10 Human tissue by using anti-CRYM monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Western Blot: mu Crystallin Antibody (OTI1G7) - Azide and BSA Free [NBP2-72825] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-CRYM monoclonal antibody (1:200).

Western Blot: mu Crystallin Antibody (OTI1G7) - Azide and BSA Free [NBP2-72825] - Analysis of extracts (35ug) from 9 different cell lines by using anti-mu Crystallin monoclonal antibody.

Immunohistochemistry: mu Crystallin Antibody (OTI1G7) - Azide and BSA Free [NBP2-72825] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-mu Crystallin mouse monoclonal antibody.

Immunohistochemistry: mu Crystallin Antibody (OTI1G7) - Azide and BSA Free [NBP2-72825] - Staining of paraffin-embedded Human breast tissue using anti-mu Crystallin mouse monoclonal antibody.

Immunohistochemistry: mu Crystallin Antibody (OTI1G7) - Azide and BSA Free [NBP2-72825] - Staining of paraffin-embedded Human colon tissue using anti-mu Crystallin mouse monoclonal antibody.

Immunohistochemistry: mu Crystallin Antibody (OTI1G7) - Azide and BSA Free [NBP2-72825] - Staining of paraffin-embedded Human Kidney tissue using anti-mu Crystallin mouse monoclonal antibody.

Immunohistochemistry: mu Crystallin Antibody (OTI1G7) - Azide and BSA Free [NBP2-72825] - Staining of paraffin-embedded Human prostate tissue using anti-mu Crystallin mouse monoclonal antibody.

Flow Cytometry: mu Crystallin Antibody (OTI1G7) - Azide and BSA Free [NBP2-72825] - HEK293T cells transfected with either RC218123 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostaining and then analyzed by flow cytometry.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1G7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

mu Crystallin Antibody (OTI1G7) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human CRYM (NP_001879) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CRYM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 33.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for mu Crystallin Antibody (OTI1G7) - Azide and BSA Free

• crystallin, mu
• DFNA40NADP-regulated thyroid-hormone binding protein
• mu-crystallin homolog
• NADP-regulated thyroid-hormone-binding protein
• THBP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRYM