mu Crystallin Antibody (OTI1G7)

Western Blot: mu Crystallin Antibody (OTI1G7) [NBP2-02829] - analysis of extracts (10ug) from 10 Human tissue by using anti-CRYM monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Immunocytochemistry/Immunofluorescence: mu Crystallin Antibody (OTI1G7) [NBP2-02829] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY mu Crystallin.

Immunohistochemistry-Paraffin: mu Crystallin Antibody (1G7) [NBP2-02829] - Staining of paraffin-embedded Human prostate tissue using anti-mu Crystallin mouse monoclonal antibody.

Flow Cytometry: mu Crystallin Antibody (OTI1G7) [NBP2-02829] - HEK293T cells transfected with either RC218123 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained and then analyzed by flow cytometry.

Western Blot: mu Crystallin Antibody (1G7) [NBP2-02829] Analysis of extracts (35ug) from 9 different cell lines by using anti-mu Crystallin monoclonal antibody.

Western Blot: mu Crystallin Antibody (1G7) [NBP2-02829] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mu Crystallin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mu Crystallin.

Western Blot: mu Crystallin Antibody (OTI1G7) [NBP2-02829] - analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-CRYM monoclonal antibody (1:200).

Immunohistochemistry-Paraffin: mu Crystallin Antibody (1G7) [NBP2-02829] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-mu Crystallin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: mu Crystallin Antibody (1G7) [NBP2-02829] - Staining of paraffin-embedded Human breast tissue using anti-mu Crystallin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: mu Crystallin Antibody (1G7) [NBP2-02829] - Staining of paraffin-embedded Human colon tissue using anti-mu Crystallin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: mu Crystallin Antibody (1G7) [NBP2-02829] - Staining of paraffin-embedded Human Kidney tissue using anti-mu Crystallin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: mu Crystallin Antibody (1G7) [NBP2-02829] - Staining of paraffin-embedded Human liver tissue using anti-mu Crystallin mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1G7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

mu Crystallin Antibody (OTI1G7) Summary

• Description: Novus Biologicals Mouse mu Crystallin Antibody (OTI1G7) (NBP2-02829) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human CRYM (NP_001879) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CRYM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 33.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for mu Crystallin Antibody (OTI1G7)

• crystallin, mu
• DFNA40NADP-regulated thyroid-hormone binding protein
• mu-crystallin homolog
• NADP-regulated thyroid-hormone-binding protein
• THBP

Background

Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRYM
• Entrez:
  • 1428(Human)