MST2/STK3 Antibody - BSA Free

Western Blot: MST2/STK3 Antibody [NBP1-32313] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7.5% SDS PAGE diluted at 1:5000

Immunocytochemistry/Immunofluorescence: MST2/STK3 Antibody [NBP1-32313] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: MST2/STK3 Antibody [NBP1-32313] - Paraffin-embedded GASTRIC CA, using antibody at 1:100 dilution.

Western Blot: MST2/STK3 Antibody [NBP1-32313] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: STK3 transfected 293T lysates 7. 5% SDS PAGE; antibody diluted at 1:5000.

• Reactivity: Hu, Mu, Rt, Bv, Ch, RM, Xp, Ze
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MST2/STK3 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human MST2/STK3. The exact sequence is proprietary.
• Localization: Cytoplasm, Nucleus
• Predicted Species: Rat (99%), Chicken (98%), Xenopus (98%), Rhesus Macaque (99%), Zebrafish (97%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STK3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (98%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for MST2/STK3 Antibody - BSA Free

• EC 2.7.11
• FLJ90748
• KRS1
• Mammalian STE20-like protein kinase 2
• Mess1
• MST2
• MST-2
• MST2EC 2.7.11.1
• MST3
• serine/threonine kinase 3 (STE20 homolog, yeast)
• serine/threonine kinase 3 (Ste20, yeast homolog)
• serine/threonine kinase 3
• serine/threonine-protein kinase 3
• Serine/threonine-protein kinase Krs-1
• STE20-like kinase MST2
• STK3

Background

Mammalian Sterile 20-Like 2 (Mst2) is most similar to the previously identified Mst1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in-vitro kinase assay indicates that Mst2 can phosphorylate an exogenous substrate, as well as itself, and phospho-amino-acid analysis indicates that it is a serine/threonine protein kinase (1) Mst2, a STE20-family member and purported Hpo ortholog, phosphorylates and activates both Lats1 and Lats2. Results indicate that Mst2-like kinases regulate Lats kinase activities in an evolutionarily conserved regulatory pathway (2). When stably expressed in HeLa cells, Mst highly sensitizes the cells to death receptor-mediated apoptosis by accelerating caspase-3 activation. These findings suggest that Mst1 and Mst2 play a role in apoptosis both upstream and downstream of caspase activation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

FAQs for MST2/STK3 Antibody (NBP1-32313). (Showing 1 - 1 of 1 FAQ).

1. Do you have any information on how this antibody was generated?
   • The immunogen used to generate this antibody does include aa 118-228; however, the immunogen is 234 aa in length and does contain regions outside of that range. Based on a blast search, this immunogen shares a 97% homology with STK4.

Bioinformatics

• Gene Symbol: STK3