MST2/STK3 Antibody

Images

 
Western Blot: MST2/STK3 Antibody [NBP1-32313] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7.5% SDS PAGE diluted at 1:5000
Immunocytochemistry/ Immunofluorescence: MST2/STK3 Antibody [NBP1-32313] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: MST2/STK3 Antibody [NBP1-32313] - Paraffin-embedded GASTRIC CA, using antibody at 1:100 dilution.
Western Blot: MST2/STK3 Antibody [NBP1-32313] - (30 ug of whole cell lysate) A: A431 B: H1299 7.5% SDS PAGE diluted at 1:500
Western Blot: MST2/STK3 Antibody [NBP1-32313] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: STK3 transfected 293T lysates 7. 5% SDS PAGE; antibody diluted at 1:5000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.84 mg/ml

Order Details

MST2/STK3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human STK3. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STK3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.84 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MST2/STK3 Antibody

  • EC 2.7.11
  • FLJ90748
  • KRS1
  • Mammalian STE20-like protein kinase 2
  • Mess1
  • MST2
  • MST-2
  • MST2EC 2.7.11.1
  • MST3
  • serine/threonine kinase 3 (STE20 homolog, yeast)
  • serine/threonine kinase 3 (Ste20, yeast homolog)
  • serine/threonine kinase 3
  • serine/threonine-protein kinase 3
  • Serine/threonine-protein kinase Krs-1
  • STE20-like kinase MST2
  • STK3

Background

Mammalian Sterile 20-Like 2 (Mst2) is most similar to the previously identified Mst1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in-vitro kinase assay indicates that Mst2 can phosphorylate an exogenous substrate, as well as itself, and phospho-amino-acid analysis indicates that it is a serine/threonine protein kinase (1) Mst2, a STE20-family member and purported Hpo ortholog, phosphorylates and activates both Lats1 and Lats2. Results indicate that Mst2-like kinases regulate Lats kinase activities in an evolutionarily conserved regulatory pathway (2). When stably expressed in HeLa cells, Mst highly sensitizes the cells to death receptor-mediated apoptosis by accelerating caspase-3 activation. These findings suggest that Mst1 and Mst2 play a role in apoptosis both upstream and downstream of caspase activation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MST2/STK3 Antibody (NBP1-32313). (Showing 1 - 1 of 1 FAQ).

  1. Do you have any information on how this antibody was generated?
    • The immunogen used to generate this antibody does include aa 118-228; however, the immunogen is 234 aa in length and does contain regions outside of that range. Based on a blast search, this immunogen shares a 97% homology with STK4.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STK3
Entrez