MST1/STK4 Antibody - BSA Free

Immunohistochemistry: STK4 Antibody [NB110-39128] - Sample: FFPE section of human placenta. Antibody: Affinity purified rabbit anti- MST1/2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Immunoprecipitation: STK4 Antibody [NB110-39128] - Detection of MST1 and MST2 on HeLa whole cell lysate using NB110-39128. MST1 and MST2 were simultaneously IPed using other MST1/2 antibodies.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MST1/STK4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MST1/STK4 Antibody - BSA Free (NB110-39128) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-MST1/STK4 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to the C-terminus of human Mammalian Sterile 20-like 1 (Serine/Threonine Kinase 4) using the numbering given in Swiss Prot entry Q13043 (GeneID 6789).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STK4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:5000-1:15000
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  MST1/STK4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MST1/STK4 Antibody - BSA Free

• kinase responsive to stress 2
• KRS2
• Mammalian STE20-like protein kinase 1
• mammalian sterile 20-like 1
• MST1
• serine/threonine-protein kinase 4
• Serine/threonine-protein kinase Krs-2
• STE20-like kinase MST1
• STK4
• YSK3

Background

MST1 and MST2 are members of the sterile 20 (Ste20)-like kinase family that acts upstream of mitogen-activated protein kinase (MAPK) pathways and regulates c-Jun N-terminal kinase (JNK), p38 and extracellular signal-regulated kinase (ERK). Ste20-like kinases are involved in a variety of cellular processes which include gene transcription, stress responses, cytoskeletal organization and apoptosis. In response to apoptotic signals, MST1 and MST2 are cleaved by caspase-3 and mediate apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STK4 Antibody (NB110-39128)(2)

Publications using NB110-39128

• Park J, Kang SI, Lee SY et al. Tumor suppressor ras association domain family 5 (RASSF5/NORE1) mediates death receptor ligand-induced apoptosis. J Biol Chem 2010-11-01 [PMID: 20810663]
• Lau YK, Murray LB, Houshmandi SS et al. Merlin Is a Potent Inhibitor of Glioma Growth. Cancer Res. 2008-07-15 [PMID: 18632626]

Bioinformatics

• Gene Symbol: STK4
• Entrez:
  • 6789(Human)
  • 58231(Mouse)
• Uniprot:
  • Q13043()