MST1/STK4 Antibody [Unconjugated]

Western blot shows lysates of human brain tissue, A172 human glioblastoma cell line, Jurkat human acute T cell leukemia cell line, and SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MST1/STK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4939) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MST1/STK4 at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

MST1/STK4 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Goat Anti-Human MST1/STK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4939) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MST1/STK4 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human MST1/STK4
  Lys285-Asp443
  Accession # Q13043
• Specificity: Detects human MST1/STK4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human MST2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: STK4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MST1/STK4 Antibody [Unconjugated]

• kinase responsive to stress 2
• KRS2
• Mammalian STE20-like protein kinase 1
• mammalian sterile 20-like 1
• MST1
• serine/threonine-protein kinase 4
• Serine/threonine-protein kinase Krs-2
• STE20-like kinase MST1
• STK4
• YSK3

Background

MST1 (mammalian sterile twenty-like 1; also Ser/Thr-protein kinase 4) is a 56 kDa class II GC (germinal center) kinase that contains an STE class catalytic domain. It is ubiquitously expressed, and found in both cytoplasm and nucleus. Human MST1 is 487 amino acids (aa) in length. It contains a Ser/Thr protein kinase domain (aa 30‑281), followed by two caspase cleavage sites (Asp326 and 349), two NLS motifs (aa 361‑370 and 441‑451) and a dimerization domain that spans the C‑terminal 57 amino acids. Upon dimerization, MST1 is activated via autophosphorylation at Thr183. Two MST1 variants exist; one shows a substitution of three Lys for the C‑terminal 85 aa, while a second shows a 27 aa substitution for the C‑terminal 55 amino acids. Over aa 285‑443, human MST1 shows 94% aa identity to mouse MST1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MST1/STK4 Antibody (AF4939)(1)

Publication using AF4939

• JR Zhao, WW Cheng, YX Wang, M Cai, WB Wu, HJ Zhang Identification of microRNA signature in the progression of gestational trophoblastic disease Cell Death Dis, 2018-01-24;9(2):94. 2018-01-24 [PMID: 29367697] (Western Blot, Human)

Bioinformatics

• Gene Symbol: STK4
• Uniprot:
  • Q13043()