MST1/STK4 Antibody [Alexa Fluor® 647]

• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

MST1/STK4 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human MST1/STK4
  Lys285-Asp443
  Accession # Q13043
• Specificity: Detects human MST1/STK4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human MST2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

MST1 (mammalian sterile twenty-like 1; also Ser/Thr-protein kinase 4) is a 56 kDa class II GC (germinal center) kinase that contains an STE class catalytic domain. It is ubiquitously expressed, and found in both cytoplasm and nucleus. Human MST1 is 487 amino acids (aa) in length. It contains a Ser/Thr protein kinase domain (aa 30‑281), followed by two caspase cleavage sites (Asp326 and 349), two NLS motifs (aa 361‑370 and 441‑451) and a dimerization domain that spans the C‑terminal 57 amino acids. Upon dimerization, MST1 is activated via autophosphorylation at Thr183. Two MST1 variants exist; one shows a substitution of three Lys for the C‑terminal 85 aa, while a second shows a 27 aa substitution for the C‑terminal 55 amino acids. Over aa 285‑443, human MST1 shows 94% aa identity to mouse MST1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.