MSK1/RPS6KA5 Antibody (252608)

MSK1 was detected in immersion fixed human lymphocytes using 8 µg/mL Human MSK1 Monoclonal Antibody (Catalog # MAB2518) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 252608
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MSK1/RPS6KA5 Antibody (252608) Summary

• Immunogen: E. coli-derived recombinant human MSK1
  Met1-Ala802
  Accession # O75582
• Specificity: Detects human MSK1 but not human MSK2 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RPS6KA5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MSK1/RPS6KA5 Antibody (252608)

• EC 2.7.11
• MGC1911,90 kDa ribosomal protein S6 kinase 5
• MSK1
• MSK1EC 2.7.11.1
• MSPK1
• Nuclear mitogen- and stress-activated protein kinase 1
• ribosomal protein S6 kinase alpha-5
• ribosomal protein S6 kinase, 90kD, polypeptide 5
• ribosomal protein S6 kinase, 90kDa, polypeptide 5
• RLPK
• RPS6KA5
• RSKL
• RSK-like protein kinase
• S6K-alpha-5

Background

Mitogen- and stress-activated protein kinase 1 (MSK1), also known as 90 kDa ribosomal protein S6 kinase 5 (RPS6KA5), belongs to the AGC family of kinases. Both MSK1 and the related MSK2 have two kinase domains connected by a regulatory linker region and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPS6KA5
• Uniprot:
  • O75582()