MSK2 [p Ser196] Antibody [Unconjugated]

MSK2/RSKB was detected in immersion fixed paraffin-embedded sections of human pancreas using Rabbit Anti-Human/Mouse Phospho-MSK2/RSKB (S196) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF189) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human HGF (Catalog # 294-HG) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-MSK2/RSKB (S196) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF189), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

MSK2 [p Ser196] Antibody [Unconjugated] Summary

• Immunogen: Phosphopeptide containing human and mouse MSK2/RSKB S196 site
• Modification: p Ser196
• Specificity: Detects human and mouse MSK2/RSKB when phosphorylated at S196.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPS6KA4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in PBS with Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Concentration: LYOPH

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MSK2 [p Ser196] Antibody [Unconjugated]

• MSK2
• ribosomal protein S6 kinase, 90kDa, polypeptide 4
• RPS6KA4
• RSKB

Background

Mitogen- and stress-activated protein kinase 1 (MSK1), also known as ribosomal protein S6 kinase 5 (RPS6KA5), belongs to the AGC family of kinases. Both MSK1 and the related MSK2, also known as RSKB and RPS6KA4, have two kinase domains connected by a regulatory linker region, and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MSK2 Antibody (AF189)(2)

Publications using AF189

• Gesser B, Johansen C, Rasmussen MK, Funding AT, Otkjaer K, Kjellerup RB, Kragballe K, Iversen L Dimethylfumarate specifically inhibits the mitogen and stress-activated kinases 1 and 2 (MSK1/2): possible role for its anti-psoriatic effect. J. Invest. Dermatol., 2007-05-10;127(9):2129-37. 2007-05-10 [PMID: 17495961] (Western Blot, Human)
• Funding AT, Johansen C, Kragballe K, Iversen L Mitogen- and stress-activated protein kinase 2 and cyclic AMP response element binding protein are activated in lesional psoriatic epidermis. J. Invest. Dermatol., 2007-04-12;127(8):2012-9. 2007-04-12 [PMID: 17429437] (IHC-P, Human)

Bioinformatics

• Gene Symbol: RPS6KA4