MSH2 Antibody (rMSH2/6548)

Western Blot: MSH2 Antibody (rMSH2/6548) [NBP3-07210] - Western blot analysis of HCT116 cell lysate using MSH2 Recombinant Mouse Monoclonal Antibody (rMSH2/6548).

Immunohistochemistry-Paraffin: MSH2 Antibody (rMSH2/6548) [NBP3-07210] - Formalin-fixed, paraffin-embedded dog lymph node stained with MSH2 Antibody (rMSH2/6548).

Immunohistochemistry-Paraffin: MSH2 Antibody (rMSH2/6548) [NBP3-07210] - Formalin-fixed, paraffin-embedded human colon stained with MSH2 Recombinant Mouse Monoclonal Antibody (rMSH2/6548).

Immunohistochemistry-Paraffin: MSH2 Antibody (rMSH2/6548) [NBP3-07210] - Formalin-fixed, paraffin-embedded human colon stained with MSH2 Recombinant Mouse Monoclonal Antibody (rMSH2/6548).

Immunohistochemistry-Paraffin: MSH2 Antibody (rMSH2/6548) [NBP3-07210] - Formalin-fixed, paraffin-embedded cat lymph node stained with MSH2 Antibody (rMSH2/6548).

• Reactivity: Hu, Ca, Fe
• Applications: WB, IHC
• Clone: rMSH2/6548
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MSH2 Antibody (rMSH2/6548) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08956)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant full-length human MSH2 protein (Uniprot: IDP43246)
• Localization: Nuclear
• Marker: DNA Mismatch Repair Marker
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MSH2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for MSH2 Antibody (rMSH2/6548)

• COCA1
• DNA mismatch repair protein Msh2
• FCC1
• hMSH2
• HNPCC
• HNPCC1
• HNPCC1mutS (E. coli) homolog 2 (colon cancer, nonpolyposis type 1)
• LCFS2
• MSH2
• mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
• MutS protein homolog 2

Background

MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MSH2