MSH2 Antibody (MSH2/2622) - Azide and BSA Free

Western Blot: MSH2 Antibody (MSH2/2622) - Azide and BSA Free [NBP3-08957] - Western Blot Analysis of human HepG2, A549, and A431 cell lysate using MSH2 Mouse Monoclonal Antibody (MSH2/2622).

Immunocytochemistry/Immunofluorescence: MSH2 Antibody (MSH2/2622) - Azide and BSA Free [NBP3-08957] - Immunofluorescence staining of MOLT-4 cells using MSH2 Mouse Monoclonal Antibody (MSH2/2622) followed by goat anti- Mouse IgG-CF488 (green). Membrane are stained with Phalloidin (Red).

Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) - Azide and BSA Free [NBP3-08957] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).

Flow Cytometry: MSH2 Antibody (MSH2/2622) - Azide and BSA Free [NBP3-08957] - Flow Cytometric Analysis of A549 cells using MSH2 Mouse Monoclonal Antibody (MSH2/2622) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) - Azide and BSA Free [NBP3-08957] - Formalin-fixed, paraffin-embedded human Colon from Lynch disease stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).

Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) - Azide and BSA Free [NBP3-08957] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).

Immunohistochemistry-Paraffin: MSH2 Antibody (MSH2/2622) - Azide and BSA Free [NBP3-08957] - Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with MSH2 Mouse Monoclonal Antibody (MSH2/2622).

• Reactivity: Hu, Ca
• Applications: WB, Flow, ICC/IF, IHC
• Clone: MSH2/2622
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

MSH2 Antibody (MSH2/2622) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07211).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant fragment (around aa 327-427) of human MSH2 protein (exact sequence is proprietary) (Uniprot: IDP43246)
• Localization: Nuclear
• Marker: DNA Mismatch Repair Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MSH2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for MSH2 Antibody (MSH2/2622) - Azide and BSA Free

• COCA1
• DNA mismatch repair protein Msh2
• FCC1
• hMSH2
• HNPCC
• HNPCC1
• HNPCC1mutS (E. coli) homolog 2 (colon cancer, nonpolyposis type 1)
• LCFS2
• MSH2
• mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
• MutS protein homolog 2

Background

MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MSH2