MRPS2 Antibody (OTI4D6) - Azide and BSA Free

Western Blot: MRPS2 Antibody (OTI4D6) - Azide and BSA Free [NBP2-72783] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS2.

Immunohistochemistry: MRPS2 Antibody (OTI4D6) - Azide and BSA Free [NBP2-72783] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MRPS2 mouse monoclonal antibody.

Flow Cytometry: MRPS2 Antibody (OTI4D6) - Azide and BSA Free [NBP2-72783] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-MRPS2 antibody, and then analyzed by flow cytometry.

Flow Cytometry: MRPS2 Antibody (OTI4D6) - Azide and BSA Free [NBP2-72783] - Analysis of Hela cells, using anti-MRPS2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: MRPS2 Antibody (OTI4D6) - Azide and BSA Free [NBP2-72783] - Analysis of Jurkat cells, using anti-MRPS2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI4D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

MRPS2 Antibody (OTI4D6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse MRPS2 Antibody (OTI4D6) - Azide and BSA Free (NBP2-03292) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human MRPS2 (NP_057118) produced in E.coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MRPS2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 33.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for MRPS2 Antibody (OTI4D6) - Azide and BSA Free

• 28S ribosomal protein S2, mitochondrial
• CGI-91
• mitochondrial 28S ribosomal protein S2
• mitochondrial ribosomal protein S2
• MRP-S2
• S2mt

Background

The Mitochondrial Ribosomal Protein S2, or MRPS2, consists of a 296 long amino acid isoform that is 33 kDa, and is involved in protein synthesis as a component of the 28S subunit on the mitochondrial ribosome. Disease research is currently being conducted on the relationship between MRPS2 and pneumonia and mycobacterium tuberculosis. MRPS2 has been linked to the process of translation, and interacts with MRPS5, ICT1, ESR2, MRPS14, and MRPS11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRPS2