MRPL12 Antibody - BSA Free

Western Blot: MRPL12 Antibody [NBP2-97472] - Anti-MRPL12 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa

Immunohistochemistry-Paraffin: MRPL12 Antibody [NBP2-97472] - Immunochemical staining of human MRPL12 in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: MRPL12 Antibody [NBP2-97472] - Immunochemical staining of human MRPL12 in human colon with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: MRPL12 Antibody [NBP2-97472] - MRPL12 was immunoprecipitated using: Lane A: 0.5 mg A431 Whole Cell Lysate 4 ul anti-MRPL12 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MRPL12 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa

Immunocytochemistry/Immunofluorescence: MRPL12 Antibody [NBP2-97472] - Immunofluorescence staining of MRPL12 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97472 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MRPL12 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human MRPL12 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRPL12
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Immunoprecipitation 0.5-2uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for MRPL12 Antibody - BSA Free

• 5c5-2
• FLJ60124
• L12mt
• MGC8610
• mitochondrial ribosomal protein L12
• MRP-L12
• MRP-L31/34
• MRPL7
• MRPL7/L12
• RPML1239S ribosomal protein L12, mitochondrial

Background

MRPL12, also known as 39 S ribosomal protein L12, mitochondrial, is a 21.3 kDa, 198 amino acid protein that is involved in mitochondrial protein synthesis by forming homodimers in 39S subunits. Current research is being conducted to identify the effect of the protein on diseases such as prostate cancer and prostatitis. The protein interacts in translation pathways with other proteins such as ICT1, MRPL3, SRRM2, PPP2R2B, and TMEM51.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRPL12