MRPL12 Antibody - BSA Free

Western Blot: MRPL12 Antibody - BSA Free [MRPL12] - Western blot analysis of various lysates using MRPL12 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.

Immunocytochemistry/ Immunofluorescence: MRPL12 Antibody - BSA Free [MRPL12] - Immunofluorescence analysis of U-2 OS cells using MRPL12 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry: MRPL12 Antibody - BSA Free [MRPL12] - Immunohistochemistry analysis of MRPL12 in paraffin-embedded human thyroid cancer tissue using MRPL12 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: MRPL12 Antibody - BSA Free [MRPL12] - Immunofluorescence analysis of L-929 cells using MRPL12 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: MRPL12 Antibody - BSA Free [MRPL12] - Immunofluorescence analysis of C6 cells using MRPL12 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MRPL12 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MRPL12 Antibody - BSA Free (NBP2-95243) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human MRPL12 (NP_002940.2). MLPAAARPLWGPCLGLRAAAFRLARRQVPCVCAVRHMRSSGHQRCEALAGAPLDNAPKEYPPKIQQLVQDIASLTLLEISDLNELLKKTLKIQDVGLVPMGGVMSGAVPAAAAQEAVEEDIPIAKERTHFTVRLTEAKPVDKVKLIKEIKNYIQGINLVQAKKLVESLPQEIKANVAKAEAEKIKAALEAVGGTVVLE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRPL12
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MRPL12 Antibody - BSA Free

• 5c5-2
• FLJ60124
• L12mt
• MGC8610
• mitochondrial ribosomal protein L12
• MRP-L12
• MRP-L31/34
• MRPL7
• MRPL7/L12
• RPML1239S ribosomal protein L12, mitochondrial

Background

MRPL12, also known as 39 S ribosomal protein L12, mitochondrial, is a 21.3 kDa, 198 amino acid protein that is involved in mitochondrial protein synthesis by forming homodimers in 39S subunits. Current research is being conducted to identify the effect of the protein on diseases such as prostate cancer and prostatitis. The protein interacts in translation pathways with other proteins such as ICT1, MRPL3, SRRM2, PPP2R2B, and TMEM51.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRPL12