MRCL3 [p Ser19] Antibody

Product Discontinued

MRCL3 [p Ser19] Antibody Summary

• Immunogen: Phospho peptide containing pSer19 (human).
• Modification: p Ser19
• Specificity: Human Myosin Light Chain phospho peptide corresponding to a region of the human smooth/non-muscle form of myosin regulatory light chain conjugated to Keyhole Limpet Hemocyanin (KLH).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYL12A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000 - 1:20000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 2 - 5 ug/ml
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:1000 - 1:5000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
• Preservative: 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MRCL3 [p Ser19] Antibody

• MLCBMyosin RLC
• MRCL3
• MRLC3MLC-2B
• MYL2B
• myosin regulatory light chain 12A
• Myosin regulatory light chain 2, nonsarcomeric
• myosin regulatory light chain 3
• myosin regulatory light chain MRCL3
• Myosin regulatory light chain MRLC3
• myosin, light chain 12A, regulatory, non-sarcomeric
• myosin, light polypeptide, regulatory, non-sarcomeric (20kD)
• RLC

Background

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium levels and myosin phosphorylation, resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. This antibody is specific for the phosphorylated form of myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human and mouse have almost identical sequences. In human the phosphorylation site is pS19, while in mouse the site maps to pS20.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYL12A
• Entrez:
  • 10627(Human)
• Uniprot:
  • P19105(Human)