MRCK Antibody (1G7) - Azide and BSA Free

Western Blot: MRCK Antibody (1G7) [H00008476-M01] - Western Blot analysis of CDC42BPA expression in A-549 ( Cat # L025V1 ).

Western Blot: MRCK Antibody (1G7) [H00008476-M01] - Western Blot analysis of CDC42BPA expression in A-549 ( Cat # L025V1 ).

Sandwich ELISA: MRCK Antibody (1G7) [H00008476-M01] - Detection limit for recombinant GST tagged CDC42BPA is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1G7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MRCK Antibody (1G7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CDC42BPA (NP_003598, 551 a.a. ~ 650 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LVQASERLKNQSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKVESLRQELRRTERAKKELEVHTEALAAEASKDRKLRE
• Specificity: CDC42BPA - CDC42 binding protein kinase alpha (DMPK-like)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDC42BPA
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MRCK Antibody (1G7) - Azide and BSA Free

• CDC42 binding protein kinase alpha (DMPK-like)
• CDC42 binidng protein kinase beta
• CDC42-binding protein kinase alpha (DMPK-like)
• CDC42-binding protein kinase alpha
• DMPK-like alpha
• EC 2.7.11
• EC 2.7.11.1
• KIAA0451DKFZp686L1738
• MRCK alpha
• MRCK
• MRCKADKFZp686P1738
• Myotonic dystrophy kinase-related CDC42-binding kinase alpha
• myotonic dystrophy kinase-related CDC42-binding protein kinase alpha
• Myotonic dystrophy protein kinase-like alpha
• PK428FLJ23347
• serine/threonine-protein kinase MRCK alpha
• ser-thr protein kinase PK428
• ser-thr protein kinase related to the myotonic dystrophy protein kinase

Background

The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MRCK Antibody (H00008476-M01)(1)

Publication using H00008476-M01

• Unbekandt M, Belshaw S, Bower J et al. Discovery of Potent and Selective MRCK Inhibitors with Therapeutic Effect on Skin Cancer. Cancer Res 2018-04-15 [PMID: 29382705]

Bioinformatics

• Gene Symbol: CDC42BPA
• Entrez:
  • 8476(Human)
• OMIM:
  • 603412(Human)
• Uniprot:
  • NP_003598(Human)