MR1 Antibody (909417) [Unconjugated]

MR1 was detected in perfusion fixed frozen sections of rat intestine using Rat Anti-Mouse MR1 Monoclonal Antibody (Catalog # MAB8526) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to villi epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: IHC
• Clone: 909417
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

MR1 Antibody (909417) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MR1
  Met1-Arg296
  Accession # Q8HWB0
• Specificity: Detects mouse MR1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: MR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MR1 Antibody (909417) [Unconjugated]

• Class I histocompatibility antigen-like protein
• FLJ31593
• H2ls
• HLALS
• major histocompatibility complex class I-related gene protein
• major histocompatibility complex, class I-like sequence
• major histocompatibility complex, class I-related
• MHC class I-like antigen MR-1
• MHC class I-related gene protein
• MHC class-I related-gene protein
• MR1

Background

The Major Histocompatibility Complex (MHC) class I-related gene, MR1, considered a non-classical MHC class IA gene which is encoded outside the MHC region. The MR1 is responsible for activation of Mucosal-associated Invariant T (MAIT) cells expressing semi-invariant T cell receptors in the presence of bacteria. MR1 is a highly evolutionary conserved protein with regard to alpha 1 and alpha 2 domains corresponding to the peptide-binding domains of classical MHC class I molecules which show about 90% amino acid identity between human and mouse. MR1 protein can associate with beta 2-microglobulin, which indicates that this is a typical class I heterodimer composed of a heavy and a light chain resembling the classical MHC class I molecules.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MR1
• Uniprot:
  • Q8HWB0()