MPDU1 Overexpression Lysate (Native)

Images

 
Western Blot: MPDU1 Overexpression Lysate (Adult Normal) [NBL1-13196] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MPDU1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MPDU1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200571 and based on accession number NM_004870. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens mannose-P-dolichol utilization defect 1 (MPDU1), transcript variant 1, mRNA.
Gene
MPDU1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
26.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MPDU1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MPDU1 Overexpression Lysate (Native)

  • CDGIF
  • FLJ14836
  • HBeAg-binding protein 2 binding protein A
  • Lec35
  • mannose-P-dolichol utilization defect 1 protein
  • mannose-P-dolichol utilization defect 1
  • My008
  • PP3958
  • PQLC5
  • SL15HBEBP2BPA
  • Suppressor of Lec15 and Lec35 glycosylation mutation homolog

Background

This gene encodes an endoplasmic reticulum membrane protein that is required for utilization of the mannose donor mannose-P-dolichol in the synthesis of lipid-linked oligosaccharides and glycosylphosphatidylinositols. Mutations in this gene result in congenital disorder of glycosylation type If. Alternative splicing results in multiple transcript variants. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-05034
Species: Hu
Applications: WB, PEP-ELISA
H00001497-M09
Species: Hu, Mu, Rt
Applications: WB, ELISA
NBP1-84597
Species: Hu
Applications: WB, IHC, IHC-P
NB100-2421
Species: Hu
Applications: WB, Flow, IHC, IHC-P, PEP-ELISA
NBP2-30375
Species: Hu
Applications: IHC, IHC-P
AF1717
Species: Hu
Applications: WB, IP
NBP2-57753
Species: Hu
Applications: WB, ICC/IF
NBP1-89111
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-02541
Species: Hu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-24557
Species: Hu, Eq, Pm
Applications: WB, IHC-P
AF1725
Species: Hu, Mu, Rt
Applications: WB, Simple Western
NBP1-62494
Species: Hu
Applications: WB
NBP2-02043
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-91658
Species: Hu
Applications: WB, IHC, IHC-P
MAB6668
Species: Hu
Applications: WB, CyTOF-ready, ICC, ICFlow
NBP1-91979
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-50179
Species: Hu
Applications: WB, Flow, Func, ICC/IF, IP, CyTOF-ready
NBP1-58906
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for MPDU1 Lysate (NBL1-13196) (0)

There are no publications for MPDU1 Lysate (NBL1-13196).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MPDU1 Lysate (NBL1-13196) (0)

There are no reviews for MPDU1 Lysate (NBL1-13196). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MPDU1 Lysate (NBL1-13196) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional MPDU1 Products

Bioinformatics Tool for MPDU1 Lysate (NBL1-13196)

Discover related pathways, diseases and genes to MPDU1 Lysate (NBL1-13196). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MPDU1 Lysate (NBL1-13196)

Discover more about diseases related to MPDU1 Lysate (NBL1-13196).
 

Pathways for MPDU1 Lysate (NBL1-13196)

View related products by pathway.

PTMs for MPDU1 Lysate (NBL1-13196)

Learn more about PTMs related to MPDU1 Lysate (NBL1-13196).

Blogs on MPDU1

There are no specific blogs for MPDU1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

MPDU1 Antibody
NBP1-84570
DARC Antibody
NB100-2421

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MPDU1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol MPDU1