Mouse SCF Quantikine ELISA Kit

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

Mouse SCF Quantikine ELISA Kit Summary

Background
The Quantikine Mouse SCF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse SCF levels in cell culture supernates, serum, and EDTA plasma. It contains E. coli-expressed recombinant mouse SCF and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse SCF showed linear curves that were parallel to the standard curves obtain...ed using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse SCF.
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Specificity
Natural and recombinant mouse SCF
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Kitl

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using MCK00.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse SCF Quantikine ELISA Kit

  • c-kit Ligand
  • DCUA
  • DFNA69
  • DKFZp686F2250
  • familial progressive hyperpigmentation 2
  • FPH2
  • FPHH
  • KIT ligand
  • Kitl
  • KITLG
  • KL-1
  • Mast cell growth factor
  • MGF
  • MGFSHEP7
  • SCF
  • SCFStem cell factor
  • SFc-Kit ligand
  • SHEP7
  • SLF
  • steel factor

Background

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail. The ECD shows both N-linked and O-linked glycosylation. Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice. An alternately spliced isoform of human SCF lacks 28 aa that encompasses the primary proteolytic recognition site. Within the ECD of the short isoform (corresponding to this recombinant protein), human SCF shares 75 - 83% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF-R/c-kit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for SCF/c-kit Ligand (MCK00)(22)

We have publications tested in 2 confirmed species: Human, Mouse.


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Human
(1)
Mouse
(21)
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Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using MCK00 Applications Species
JC Huang, SC Chen, WA Chang, WW Hung, PH Wu, LY Wu, JM Chang, YL Hsu, YC Tsai KITLG Promotes Glomerular Endothelial Cell Injury in Diabetic Nephropathy by an Autocrine Effect International Journal of Molecular Sciences, 2022;23(19):. 2022 [PMID: 36233032] (Human) Human
Y Omatsu, S Aiba, T Maeta, K Higaki, K Aoki, H Watanabe, G Kondoh, R Nishimura, S Takeda, UI Chung, T Nagasawa Runx1 and Runx2 inhibit fibrotic conversion of cellular niches for hematopoietic stem cells Nature Communications, 2022;13(1):2654. 2022 [PMID: 35551452] (Mouse) Mouse
BM Foster, KL Langsten, A Mansour, L Shi, BA Kerr Tissue distribution of stem cell factor in adults Experimental and molecular pathology, 2021;122(0):104678. 2021 [PMID: 34450114] (Mouse) Mouse
AA Hastreiter, GG Dos Santos, EN Makiyama, EWC Santos, P Borelli, RA Fock Effects of protein malnutrition on haematopoietic regulatory activity of bone marrow mesenchymal stem cells The Journal of nutritional biochemistry, 2021;0(0):108626. 2021 [PMID: 33705953] (Mouse) Mouse
S Sinha, M Biswas, SS Chatterjee, S Kumar, A Sengupta Pbrm1 Steers Mesenchymal Stromal Cell Osteolineage Differentiation by Integrating PBAF-Dependent Chromatin Remodeling and BMP/TGF-&beta Signaling Cell Rep, 2020;31(4):107570. 2020 [PMID: 32348751] (Mouse) Mouse
W Fonseca, AJ Rasky, C Ptaschinsk, SH Morris, SKK Best, M Phillips, CA Malinczak, NW Lukacs Group 2 innate lymphoid cells (ILC2) are regulated by stem cell factor during chronic asthmatic disease Mucosal Immunol, 2019;12(2):445-456. 2019 [PMID: 30617299] (Mouse) Mouse
M Mastri, CR Lee, A Tracz, RS Kerbel, M Dolan, Y Shi, JML Ebos Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors Mol. Cancer Ther., 2018;0(0):. 2018 [PMID: 29695634] (Mouse) Mouse
First steps to define murine amniotic fluid stem cell microenvironment Sci Rep, 2016;6(0):37080. 2016 [PMID: 27845396] (Mouse) Mouse
Cunha M, Lima F, Vinolo M, Hastreiter A, Curi R, Borelli P, Fock R Protein malnutrition induces bone marrow mesenchymal stem cells commitment to adipogenic differentiation leading to hematopoietic failure. PLoS ONE, 2013;8(3):e58872. 2013 [PMID: 23516566] (Mouse) Mouse
Arcangeli ML, Frontera V, Bardin F, Obrados E, Adams S, Chabannon C, Schiff C, Mancini SJ, Adams RH, Aurrand-Lions M JAM-B regulates maintenance of hematopoietic stem cells in the bone marrow. Blood, 2011;118(17):4609-19. 2011 [PMID: 21868569] (Mouse) Mouse
Show All 22 Publications.

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