Mouse SCF Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse SCF Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse SCF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse SCF levels in cell culture supernates, serum, and EDTA plasma. It contains E. coli-expressed recombinant mouse SCF and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse SCF showed linear curves that were parallel to the standard curves obtain...ed using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse SCF.
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• Specificity: Natural and recombinant mouse SCF
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: KITLG

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse SCF Quantikine ELISA Kit

• c-kit Ligand
• DCUA
• DFNA69
• DKFZp686F2250
• familial progressive hyperpigmentation 2
• FPH2
• FPHH
• KIT ligand
• Kitl
• KITLG
• KL-1
• Mast cell growth factor
• MGF
• MGFSHEP7
• SCF
• SCFStem cell factor
• SFc-Kit ligand
• SHEP7
• SLF
• steel factor

Background

Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail. The ECD shows both N-linked and O-linked glycosylation. Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice. An alternately spliced isoform of human SCF lacks 28 aa that encompasses the primary proteolytic recognition site. Within the ECD of the short isoform (corresponding to this recombinant protein), human SCF shares 75 - 83% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF-R/c-kit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for SCF/c-kit Ligand (MCK00)(27)

Publications using MCK00

• Takeishi, S;Marchand, T;Koba, WR;Borger, DK;Xu, C;Guha, C;Bergman, A;Frenette, PS;Gritsman, K;Steidl, U; Haematopoietic stem cell numbers are not solely determined by niche availability bioRxiv : the preprint server for biology 2023-10-29 [PMID: 37961493] (Transgenic Mouse, Mouse)
• Molfetta, R;Lecce, M;Milito, ND;Putro, E;Pietropaolo, G;Marangio, C;Scarno, G;Moretti, M;De Smaele, E;Santini, T;Bernardini, G;Sciumè, G;Santoni, A;Paolini, R; SCF and IL-33 regulate mouse mast cell phenotypic and functional plasticity supporting a pro-inflammatory microenvironment Cell death & disease 2023-09-20 [PMID: 37730723] (Mouse)
• Lee, HJ;Lee, J;Yang, MJ;Kim, YC;Hong, SP;Kim, JM;Hwang, GS;Koh, GY; Endothelial cell-derived stem cell factor promotes lipid accumulation through c-Kit-mediated increase of lipogenic enzymes in brown adipocytes Nature communications 2023-05-13 [PMID: 37179330] (Mouse)
• SY Heazlewood, T Ahmad, B Cao, H Cao, M Domingues, X Sun, CK Heazlewood, S Li, B Williams, M Fulton, JF White, T Nebl, CM Nefzger, JM Polo, BT Kile, F Kraus, MT Ryan, YB Sun, PFM Choong, SL Ellis, ML Anko, SK Nilsson High ploidy large cytoplasmic megakaryocytes are hematopoietic stem cells regulators and essential for platelet production Nature Communications, 2023-04-13;14(1):2099. 2023-04-13 [PMID: 37055407] (Mouse)
• M Shah, H Kumar, S Qiu, H Li, M Harris, J He, A Abraham, DK Crossman, A Paterson, RS Welner, R Bhatia Low c-Kit expression identifies primitive therapy-resistant CML stem cells JCI Insight, 2023-01-10;0(0):. 2023-01-10 [PMID: 36413413] (Mouse)
• JC Huang, SC Chen, WA Chang, WW Hung, PH Wu, LY Wu, JM Chang, YL Hsu, YC Tsai KITLG Promotes Glomerular Endothelial Cell Injury in Diabetic Nephropathy by an Autocrine Effect International Journal of Molecular Sciences, 2022-10-03;23(19):. 2022-10-03 [PMID: 36233032] (Human, Mouse)
• Y Omatsu, S Aiba, T Maeta, K Higaki, K Aoki, H Watanabe, G Kondoh, R Nishimura, S Takeda, UI Chung, T Nagasawa Runx1 and Runx2 inhibit fibrotic conversion of cellular niches for hematopoietic stem cells Nature Communications, 2022-05-12;13(1):2654. 2022-05-12 [PMID: 35551452] (Mouse)
• BM Foster, KL Langsten, A Mansour, L Shi, BA Kerr Tissue distribution of stem cell factor in adults Experimental and molecular pathology, 2021-08-24;122(0):104678. 2021-08-24 [PMID: 34450114] (Mouse)
• AA Hastreiter, GG Dos Santos, EN Makiyama, EWC Santos, P Borelli, RA Fock Effects of protein malnutrition on haematopoietic regulatory activity of bone marrow mesenchymal stem cells The Journal of nutritional biochemistry, 2021-03-08;0(0):108626. 2021-03-08 [PMID: 33705953] (Mouse)
• S Sinha, M Biswas, SS Chatterjee, S Kumar, A Sengupta Pbrm1 Steers Mesenchymal Stromal Cell Osteolineage Differentiation by Integrating PBAF-Dependent Chromatin Remodeling and BMP/TGF-&beta Signaling Cell Rep, 2020-04-28;31(4):107570. 2020-04-28 [PMID: 32348751] (Mouse)
• W Fonseca, AJ Rasky, C Ptaschinsk, SH Morris, SKK Best, M Phillips, CA Malinczak, NW Lukacs Group 2 innate lymphoid cells (ILC2) are regulated by stem cell factor during chronic asthmatic disease Mucosal Immunol, 2019-01-07;12(2):445-456. 2019-01-07 [PMID: 30617299] (Mouse)
• M Mastri, CR Lee, A Tracz, RS Kerbel, M Dolan, Y Shi, JML Ebos Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors Mol. Cancer Ther., 2018-04-25;0(0):. 2018-04-25 [PMID: 29695634] (Mouse)
• First steps to define murine amniotic fluid stem cell microenvironment Sci Rep, 2016-11-15;6(0):37080. 2016-11-15 [PMID: 27845396] (Mouse)
• Cunha M, Lima F, Vinolo M, Hastreiter A, Curi R, Borelli P, Fock R Protein malnutrition induces bone marrow mesenchymal stem cells commitment to adipogenic differentiation leading to hematopoietic failure. PLoS ONE, 2013-03-14;8(3):e58872. 2013-03-14 [PMID: 23516566] (Mouse)
• Arcangeli ML, Frontera V, Bardin F, Obrados E, Adams S, Chabannon C, Schiff C, Mancini SJ, Adams RH, Aurrand-Lions M JAM-B regulates maintenance of hematopoietic stem cells in the bone marrow. Blood, 2011-08-25;118(17):4609-19. 2011-08-25 [PMID: 21868569] (Mouse)
• Horiuchi K, Morioka H, Takaishi H, Akiyama H, Blobel CP, Toyama Y Ectodomain shedding of FLT3 ligand is mediated by TNF-alpha converting enzyme. J. Immunol., 2009-06-15;182(12):7408-14. 2009-06-15 [PMID: 19494263] (Mouse)
• Suh HC, Ji M, Gooya J, Lee M, Klarmann KD, Keller JR Cell-nonautonomous function of Id1 in the hematopoietic progenitor cell niche. Blood, 2009-05-28;114(6):1186-95. 2009-05-28 [PMID: 19478045] (Mouse)
• Schroeter MR, Humboldt T, Schafer K, Konstantinides S Rosuvastatin reduces atherosclerotic lesions and promotes progenitor cell mobilisation and recruitment in apolipoprotein E knockout mice. Atherosclerosis, 2008-11-25;205(1):63-73. 2008-11-25 [PMID: 19111832] (Mouse)
• Higuchi K, Ayach B, Sato T, Chen M, Devine SP, Rasaiah VI, Dawood F, Yanagisawa T, Tei C, Takenaka T, Liu PP, Medin JA Direct injection of kit ligand-2 lentivirus improves cardiac repair and rescues mice post-myocardial infarction. Mol. Ther., 2008-11-11;17(2):262-8. 2008-11-11 [PMID: 19002160] (Mouse)
• Huang B, Lei Z, Zhang GM, Li D, Song C, Li B, Liu Y, Yuan Y, Unkeless J, Xiong H, Feng ZH SCF-mediated mast cell infiltration and activation exacerbate the inflammation and immunosuppression in tumor microenvironment. Blood, 2008-06-04;112(4):1269-79. 2008-06-04 [PMID: 18524989] (Mouse)
• Zhang P, Nelson S, Bagby GJ, Siggins R, Shellito JE, Welsh DA The lineage-c-Kit+Sca-1+ cell response to Escherichia coli bacteremia in Balb/c mice. Stem Cells, 2008-05-15;26(7):1778-86. 2008-05-15 [PMID: 18483422] (Mouse)
• Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy. Proc. Natl. Acad. Sci. U.S.A., 2007-10-17;104(43):17069-74. 2007-10-17 [PMID: 17942672] (Mouse)
• Pan PY, Wang GX, Yin B, Ozao J, Ku T, Divino CM, Chen SH Reversion of immune tolerance in advanced malignancy: modulation of myeloid-derived suppressor cell development by blockade of stem-cell factor function. Blood, 2007-09-20;111(1):219-28. 2007-09-20 [PMID: 17885078] (Mouse)
• Wang CH, Verma S, Hsieh IC, Chen YJ, Kuo LT, Yang NI, Wang SY, Wu MY, Hsu CM, Cheng CW, Cherng WJ Enalapril increases ischemia-induced endothelial progenitor cell mobilization through manipulation of the CD26 system. J. Mol. Cell. Cardiol., 2006-05-05;41(1):34-43. 2006-05-05 [PMID: 16678199] (Mouse)
• Liao HF, Chen YJ, Yang YC A novel polysaccharide of black soybean promotes myelopoiesis and reconstitutes bone marrow after 5-flurouracil- and irradiation-induced myelosuppression. Life Sci., 2005-02-25;77(4):400-13. 2005-02-25 [PMID: 15894009] (Mouse)
• Heissig B, Hattori K, Dias S, Friedrich M, Ferris B, Hackett NR, Crystal RG, Besmer P, Lyden D, Moore MA, Werb Z, Rafii S Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand. Cell, 2002-05-31;109(5):625-37. 2002-05-31 [PMID: 12062105] (Mouse)
• Shih CC, Hu MC, Hu J A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells. Blood, 2000-03-15;95(6):1957-66. 2000-03-15 [PMID: 10706861] (Mouse)

FAQs for SCF/c-kit Ligand (MCK00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: KITLG